Real-Time PCR

Real-time PCR is a laboratory method that amplifies a target DNA or RNA sequence and measures the product as it accumulates, cycle by cycle, using a fluorescent signal. The technique, also called quantitative PCR or qPCR, reports the starting amount of template rather than only confirming its presence, which is what separates it from endpoint PCR. The Questpair catalogue lists 660 real-time PCR systems from 16 verified suppliers and distributors across 10 countries. New, used and refurbished instruments appear side by side. 98 percent of the listings are used. Prices run from EUR 58 to EUR 172.178, a spread that tracks channel count, block format and detection optics more than anything else.