Bio-Rad iQ-Check Prep System 359-4911 with CFX96 Touch Real-Time PCR Automation Solution

The Bio-Rad iQ-Check Prep System 359-4911 is an automated robotic liquid handling platform tailored for high-throughput PCR sample preparation, predominantly used in food safety molecular diagnostics and biotechnology applications. Integrated with the CFX96 Touch Real-Time PCR Detection System, this solution supports precise DNA extraction, PCR plate setup, and multiplex real-time PCR amplification necessary for foodborne pathogen detection such as *Salmonella*, *Listeria*, and *E. coli*.

• Automated Liquid Handling:
• SPAN-4 pipetting head with 1–1000 µL volume range (low volume 50 µL, high volume 1000 µL)
• Positional accuracy XYZ: 0.1 mm
• Capacitive and pressure liquid level detection for precise aspiration and dispensing
• CO-RE gripper attachments for reliable labware transfer

• PCR Amplification and Detection (CFX96 Touch System):
• C1000 Touch Thermal Cycler module with Peltier heating/cooling; ramp rate up to 5 °C/sec
• Temperature range: 0–100 °C; accuracy ±0.2 °C at 90 °C; well-to-well uniformity ±0.4 °C
• 96-well sample capacity; recommended sample volume 10–25 µL
• Optical module features 6 excitation LEDs and 6 filtered photodiodes covering 450–730 nm wavelengths
• Sensitivity to detect as low as a single copy of target DNA, dynamic range spanning 10 orders of magnitude

• Additional Integrated Components:
• Hamilton Heater Shaker for controlled temperature and agitation during sample processing
• 2mag MIXControl MS4 magnetic stirrer with four pads for reagent mixing
• Honeywell MS7120 barcode scanner for sample traceability and LIMS integration
• Supplied with Bio-Rad tube adapters compatible with iQ-Check Reagent Kits

• System Specifications:
• Dimensions (W x D x H): 1124 x 795 x 903 mm (44.25 x 31.30 x 35.55 in)
• Weight: 120 kg (265 lbs)

This combined platform is essential for molecular diagnostics workflows requiring automated PCR preparation and multiplex real-time PCR analysis, widely adopted in biotechnological research, food safety laboratories, and biopharmaceutical production pipelines. The system ensures reproducibility, traceability via integrated barcode scanning, and seamless LIMS connectivity, supporting critical bioprocessing and synthetic biology applications related to pathogen detection and gene quantification.

Ideal for medium- to high-throughput testing environments, the Bio-Rad iQ-Check Prep System reduces manual errors, accelerates sample processing, and enhances data quality in PCR-based molecular diagnostics. This solution facilitates routine foodborne pathogen screening, molecular cloning workflows, and supports translational research in biotechnology and clinical microbiology.

Keywords incorporated include: automated liquid handling system, real-time PCR detection system, food pathogen molecular diagnostics, high-throughput PCR automation, biotechnology research instrumentation, bioprocessing PCR workflows, molecular biology robot, synthetic biology PCR automation, multiplex PCR analysis, biomedical laboratory equipment.