Bio-Rad QX600 AutoDG Droplet Digital PCR System with Plate Sealer and PTC Tempo Thermal Cycler

The Bio-Rad QX600 AutoDG Droplet Digital PCR System is a fully integrated platform designed for automated droplet generation, precise digital PCR quantification, and reliable thermal cycling, optimized for advanced molecular diagnostics and biotechnology workflows. The system includes:

• Automated Droplet Generator (AutoDG) supporting 1-96 samples per run with droplet counts of approximately 20,000/sample using 20 µL starting volumes, essential for bioprocessing and gene editing research requiring uniform droplet formation.
• Droplet Reader, capable of absolute quantification of up to 12 targets per well with six fluorescence detection channels (FAM/EvaGreen, HEX/VIC, Cy5, Cy5.5, ROX, ATTO 590), enabling multiplex analysis critical for synthetic biology and molecular cloning workflows.
• PTC Tempo Deepwell Thermal Cycler featuring automated lid, gradient capability (1-24 °C programmable over 8 rows), temperature range from 4 to 100 °C, and ramp rates averaging 2 °C/sec, suitable for precise amplification protocols integrating with high-throughput droplet digital PCR applications.
• PX1 PCR Plate Sealer, providing rapid heat sealing (under 3 minutes) across a temperature range of 100-190 °C, ensuring sample integrity for downstream fluorescence analysis and minimizing evaporation during cycling.
• Complete with accompanying computer and Bio-Rad software facilitating streamlined workflow control and data analysis.

This system is widely adopted in molecular biology, clinical diagnostics, and biomanufacturing workflows for applications such as:

• Absolute DNA/RNA quantification with sensitivity down to 0.1%
• Detection of rare mutations and low-abundance variants
• Copy number variation (CNV) analysis
• Quantitative viral load measurements in biopharmaceutical production pipelines

The QX600 AutoDG system reduces hands-on time via automated droplet processing and thermal cycling, enhancing reproducibility and minimizing variability inherent to manual methods. Its advanced detection technology utilizing avalanche photodiodes and LEDs supports robust fluorescence-based measurements, crucial for live-cell imaging assay development and fluorescence analysis in biotechnology research.

Overall, the Bio-Rad QX600 AutoDG system serves as a robust solution for labs focusing on high-throughput droplet digital PCR workflows, providing consistent, reliable nucleic acid quantification essential in biotechnology, molecular diagnostics, and bioprocess development.

Key Features:

• Automated droplet generation for consistent droplet size and uniformity
• Six-channel fluorescence detection enabling multiplex target analysis
• High-throughput capacity: processes up to 96 samples per run
• Gradient-enabled deepwell thermal cycling optimized for digital PCR
• Rapid and reliable PCR plate sealing for enhanced assay consistency
• Integrated system software allowing streamlined operation and data management

This system is indispensable for researchers engaged in molecular diagnostics, gene editing studies, synthetic biology, and biopharmaceutical quality control, providing precise digital PCR quantification foundational to modern biotechnology and clinical research.