Bio-Rad CFX96 Connect Real-Time PCR System with Gradient Thermal Control and Multiplex Detection

The Bio-Rad CFX96 Connect Real-Time PCR System is a 96-well quantitative PCR thermal cycler designed for high-precision molecular biology, genomics, and clinical research applications. It utilizes six independently controlled Peltier zones ensuring exceptional temperature uniformity of ±0.4°C, critical for reproducible amplification across all wells. The system supports a temperature range of 0–100°C with an accuracy of ±0.2°C at 90°C, and features a fast ramp rate of up to 5°C per second, enhancing assay throughput and efficiency.

Equipped with a programmable thermal gradient spanning 1–24°C, it enables rapid optimization of annealing temperatures, essential for assay development and protocol optimization in gene expression analysis, genotyping, copy number variation studies, and pathogen detection. The instrument’s optical detection system comprises 3 filtered LEDs and photodiodes, providing high fluorophore sensitivity with minimal spectral cross-talk, facilitating robust multiplex qPCR assays that conserve sample and improve data throughput in biotechnology and molecular diagnostics workflows.

The system is controlled via the intuitive CFX Maestro software, providing streamlined experiment setup, real-time monitoring, and comprehensive data analysis. Its design supports diverse applications including synthetic biology research, molecular cloning, bioprocessing, and biopharmaceutical production pipelines. This platform ensures consistent, repeatable performance suitable for both routine QC and complex high-throughput screening, making it widely adopted in biomedical engineering, clinical diagnostics, and molecular biology research.

Key Technical Specifications:

• Maximum ramp rate: 5°C/sec
• Average ramp rate: 3.3°C/sec
• Heating/Cooling: Peltier technology
• Heated lid temperature: Up to 105°C
• Temperature uniformity: ±0.4°C well-to-well within 10 sec at 90°C
• Gradient range: 30–100°C with programmable 1–24°C span
• Optical detection: 3 filtered LEDs and photodiodes for multiplex fluorescence
• Compatible with standard 96-well plates

This system is essential for molecular cloning workflows and quantitative gene expression analysis, providing reliable amplification and sensitive fluorescence detection in biotechnological research and clinical genomics. The Bio-Rad CFX96 Connect is a critical instrument to support synthetic biology experiments, molecular diagnostics assays, and biomanufacturing quality control.

Included accessories encompass the 96-well optical module, CFX Maestro software pre-installed on a laptop, a communication cable, system validation report, user manual, and standard power cord. Note that users must supply appropriate spectral dye kits and perform validation upon installation.

This Bio-Rad CFX96 Connect unit has been professionally tested under operational conditions and is ready for deployment in advanced molecular biology laboratories seeking robust, high-throughput qPCR capabilities.