Thermo Fisher Applied Biosystems StepOne Plus Real-Time PCR System with 96-Well Format and 4-Color Optical Detection

The Thermo Fisher Applied Biosystems StepOne Plus is a high-precision real-time PCR system designed for molecular biology workflows, clinical diagnostics, and biotechnology research. It features a 96-well standard format with a multicolor 4-channel optical detection system (FAM/SYBR, VIC/JOE, NED/TAMRA, ROX), enabling multiplex PCR assays critical for gene expression profiling, genotyping, and molecular diagnostics.

Key technical specifications include:

• VeriFlex Peltier thermal block with 6 independent zones, providing exceptional temperature uniformity (±0.25 °C) essential in bioprocessing and synthetic biology applications.
• Dual cycling modes: fast cycling (<40 minutes at up to 2.2 °C/s) for rapid turnaround, or standard mode (<2 hours at up to 1.6 °C/s) for enhanced assay flexibility.
• Standalone operation through an intuitive touchscreen interface or remote control via LAN network connectivity.
• Proprietary StepOne software supporting relative quantification (ΔΔCt), standard curve analysis, high-resolution melting (HRM), and genotyping assays—making it critical in molecular diagnostics and gene editing studies.
• Low power consumption and compact footprint (approx. 24 kg), suitable for modern laboratories focused on biotechnology and biomedical engineering research.

This real-time PCR instrument is widely adopted in molecular cloning workflows, molecular diagnostics, and biomanufacturing pipelines requiring precise thermal cycling and multiplex fluorescence detection. It supports a broad range of applications including live-cell genetic analysis, synthetic biology research, and fluorescence-based mutation detection assays.

Equip your laboratory with this versatile and reliable system to enhance data reproducibility and throughput in advanced gene expression analysis and clinical research.