Thermo Scientific PikoReal 24 Real-Time PCR System with Multiplex qPCR and 24-Well Block

The Thermo Scientific PikoReal 24 Real-Time PCR System is a specialized quantitative PCR thermal cycler tailored for *low sample throughput* applications with its 24-well format block. This system supports advanced multiplex PCR workflows critical in molecular diagnostics, gene editing assays, and biotechnology research including molecular cloning and synthetic biology.

Key technical features include:

• Thermal block: 24 wells compatible with low profile strip tubes and PCR tubes, accommodating sample volumes from 10 to 50 μl.
• Temperature control: High precision with ±0.2°C accuracy and uniformity up to ±0.15°C; heating rates >5°C/sec and cooling rates 4.5°C/sec enable efficient cycle times.
• Heated lid system: Automatic temperature control (50–110°C) and pressing setting ensure condensation prevention and sample integrity.
• Optical detection: Utilizes a CCD detector paired with 5 excitation LEDs covering 475–640 nm excitation range and 520–740 nm emission range, supporting up to 5 detection channels for hydrolysis probe chemistries such as 5’Nuclease/TaqMan assays.
• Multiplexing capacity: Up to 4 targets can be simultaneously detected, enabling complex gene expression and allelic discrimination studies necessary in biopharmaceutical and synthetic biology pipelines.
• Analysis modules: Features absolute and relative quantification, melting curve analysis, allelic discrimination, and High Resolution Melting (HRM) analysis using robust algorithms that do not require external reference dyes.
• Control and software: Offers standalone operation with USB protocol uploads or PC control via PikoReal software allowing remote online monitoring for streamlined real-time data acquisition and experiment management.

Compact and lightweight (9.2 kg; 300 x 230 x 310 mm), the PikoReal 24 Real-Time PCR System is engineered for precision in bioprocessing, clinical diagnostics, and biomanufacturing workflows, widely adopted in live-cell gene expression profiling and fluorescence-based molecular assays. Its combination of thermal accuracy, sensitivity (down to 1 copy detection), and multiplex capacity make it an essential instrument for cutting-edge biotechnology research and molecular biology laboratories focused on molecular diagnostics and genetic analysis.