Applied Biosystems 7900HT Fast Real-Time PCR System

The Applied Biosystems 7900HT Fast Real-Time PCR System is a high-throughput, real-time quantitative PCR instrument designed for the detection and quantification of nucleic acid sequences. This system offers flexibility with interchangeable thermal cycling block formats, accommodating both 96- and 384-well plates, as well as the TaqMan™ Low Density Array. An optional Fast PCR capability reduces run times to approximately 35 minutes for a standard 96-well format and about 55 minutes for a 384-well plate.

Key features include:

• Continuous wavelength detection from 500-660 nm, enabling the use of multiple fluorophores in a single reaction.
• High-throughput compatibility, processing over 5,000 real-time PCR reactions per day with the 384-well block and automation accessory.
• Hands-free plate-loading and unloading, providing true walkaway automation to enhance laboratory productivity.
• Optional Enterprise edition software, offering data analysis tools that support 21 CFR Part 11 guidelines for data integrity and security.

This instrument is widely utilised in molecular biology, genomics, and biotechnology research for applications such as gene expression analysis, pathogen detection, and single nucleotide polymorphism (SNP) genotyping. It is commonly found in university laboratories, biomedical research centres, and clinical research facilities. The 7900HT system was discontinued in 2012; however, it remains in use in various research settings.