53k+ listingsShips to NLResponse within one hour+31 73 711 4717info@questpair.com
Procurable · sourced on request

Applied Biosystems 7500

UsedApplied BiosystemsPCR equipmentNetherlands
Procurable

Not in stock, available to order through our sourcing team

Not in stock, but we can get it for you

We source this model through our supplier network. Request a quote and we'll come back within one hour.

  1. Request a quote
  2. We confirm price and lead time
  3. You approve, we ship to you
Reaction Volume Range
20–100 µL
Calibrated Dye
SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
Dynamic Range
9 logs
Sensitivity
1 copy of RNase P gene (human genomic DNA)
Optics
CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters
Availability
Procurable
Lead time
Confirmed with your quote
All 26 specifications
  • Ships to NL
  • Response within 1 hour
  • Warranty available. Buy without worry
  • Support from a scientist

Specifications 26

Brands
Applied Biosystems
New/Used
Used
Dimensions
340 x 450 x 490 mm
Weight
34 kg
Reaction Volume Range
20–100 µL
Calibrated Dye
SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
Dynamic Range
9 logs
Sensitivity
1 copy of RNase P gene (human genomic DNA)
Optics
CCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters
Passive Reference Dye
ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Temperature Range
4 to 100°C
Temperature Accuracy
±0.25°C (35°C to 95°C after 3 min.)
Temperature Uniformity
±0.50°C (after 30 s)
Thermal Accuracy
±0.25°C (35°C to 95°C after 3 min.)
Thermal Uniformity
±0.50°C (after 30 s)
Sample Ramp Rate
±1.1°C/s
Peak Block Ramp Rate
2.5°C/s
Throughput
4 to 5 96-well plates per 8-hr day
Precision
99.7% Confidence (5000 & 10000 Copies)
Thermal Cycling System
Peltier-based System
Electrical Requirements
950 W
Voltage
120 V
External Computer
Notebook: Intel Core 2 Duo T5500 (1.66 GHz), 8X DVD, 80 GB HD, 1.0 G DDR2-533 MHz SDRAM
Software Compatibility
Primer Express, 21 CFR Part 11 Module (optional), 7500 System Software (RQ included)
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Real-time PCR system with five-colour detection and advanced multiplexing for precise nucleic acid quantification.

The Applied Biosystems 7500 Real-Time PCR System is a versatile and reliable instrument designed for quantitative PCR applications across various scientific disciplines. This system enables precise detection and quantification of nucleic acids, facilitating critical research in fields such as molecular biology, genetics, and biomedical diagnostics.

Key features include:

  • Five-colour detection system calibrated for a broad range of dyes, including FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/Cy3™, ROX™/Texas Red™, and Cy5™ dyes, enhancing multiplexing capabilities.
  • Specialised optical system that allows easy and accurate calibration to new dyes without the need for additional filter sets.
  • Advanced multi-component algorithm minimising spectral crosstalk, ensuring superior performance in multiplex assays.
  • User-friendly software featuring plate setup wizards, multi-plate data viewing capabilities, and advanced analysis tools to streamline data processing.

This real-time PCR system is widely utilised in university laboratories, biomedical research centres, and clinical environments for applications such as gene expression analysis, pathogen quantitation, and SNP genotyping. Its robust design and comprehensive software support make it a preferred choice for researchers seeking reliable and efficient quantitative PCR solutions.

The Applied Biosystems 7500 Real-Time PCR System was first introduced in 2005 and continues to be relevant in the scientific community. However, please note that the discontinuation of sales for the 7500 and 7500 Fast real-time PCR systems for research use is scheduled to begin on October 31, 2025.

Why Questpair exists

Lab equipment markets are fragmented. Buyers spend weeks calling distributors, comparing quotes, and verifying supplier reliability. Sellers struggle to reach global research labs.

We built Questpair to fix this. Every supplier on the platform is verified before listing. Every quote you receive comes from a real, vetted seller. AI prepares your case in minutes, then a real specialist takes over.

We're an independent team based in The Netherlands, working with research labs and procurement teams across Europe and worldwide.

Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

Reply in one hour

Can't find the exact configuration?

Tell us what you need and a scientist finds it for you, usually within the hour.

Last updated 4 November 2026