Thermo ABI QuantStudio 6 Pro Real-Time PCR System with 96-Well 0.2 mL Block

The Thermo ABI QuantStudio 6 Pro is a high-precision real-time PCR system designed for quantitative PCR and molecular diagnostics applications in biotechnology, clinical research, and biomedical engineering. This instrument features a 96-well 0.2 mL interchangeable thermal cycler block with an operational reaction volume range of 10–100 µL and a rapid temperature ramp rate of up to 6.5 °C/sec (average 3.66 °C/sec), enabling shortened run times (~30 minutes).

Key specifications and features include:

• Multiplex PCR capabilities allowing simultaneous detection of up to 5–6 targets per reaction, supporting advanced gene expression, mutation scanning, and microbial pathogen detection workflows.
• A broad excitation/emission wavelength range (450–600 nm excitation; 500–640 nm emission) achieved via a bright white LED light source and 5 filter/color combinations.
• High detection sensitivity with dynamic range spanning 10 orders of magnitude and the ability to detect down to a single copy, essential for applications in molecular cloning, gene editing validation, and bioprocessing quality controls.
• Robust temperature uniformity (±0.4 °C) and wide temperature range (4–99.9 °C) with Peltier-based heating/cooling ensuring consistent PCR amplification conditions.
• Advanced CMOS-based whole-plate imaging technology for precise fluorescence acquisition across all wells.
• Comprehensive dye compatibility including FAM, SYBR Green I, VIC, JOE, HEX, TET, ABY, NED, TAMRA, Cy3, JUN, ROX, Texas Red, Mustang Purple, Cy5, LIZ, and Cy5.5, facilitating diverse assay designs in synthetic biology and molecular diagnostics.

The instrument is delivered with essential accessories including a dedicated laptop with Design & Analysis software, communication and power cables, and a heated cover for optimal sample temperature control. Optional block formats (96-well 0.1 mL fast block, 384-well block) provide flexibility for varied experimental throughput.

Applications:

• Quantitative PCR (qPCR) and High-Resolution Melting (HRM) analysis
• Gene expression profiling and genotyping
• Pathogen detection, viral load quantification, and drug resistance studies
• MicroRNA analysis and epigenetic methylation studies
• Validation workflows for next-generation sequencing (NGS) data
• Protein-DNA interaction assays such as ChIP-qPCR

This system is widely adopted in multidisciplinary research fields including genomics, biotechnology, synthetic biology, and clinical diagnostics laboratories conducting biomanufacturing quality assurance and molecular diagnostics.

Keywords: real-time PCR system, quantitative PCR instrument, multiplex PCR, molecular diagnostics equipment, gene expression analysis, synthetic biology PCR platform, bioprocessing PCR instrument, clinical infectious disease testing, fluorescence qPCR detection, thermal cycler with 96-well block.

This pre-owned unit undergoes rigorous in-house servicing and calibration to ensure optimal performance, includes warranty options, remote setup, and training support, making it a reliable instrument for advanced molecular biology workflows in research and biopharma laboratories.