Agilent xCELLigence RTCA eSight Real-Time Cell Analysis, Live-Cell Imaging and Impedance System

Agilent xCELLigence RTCA eSight System

The Agilent xCELLigence RTCA eSight is a real-time cell analysis system that integrates label-free impedance measurement with brightfield and fluorescence live-cell imaging. It is designed for continuous monitoring of cell behavior, morphology, viability, proliferation, adhesion, receptor signaling, immunotherapy, and cell-killing assays.

The platform supports biotechnology, cell biology, drug discovery, immuno-oncology, and live-cell imaging workflows requiring simultaneous electrical and optical characterization.

System configuration

• Three cradles for Biosensor E-Plate 96, for impedance measurement only, or Biosensor E-Plate View 96, for impedance measurement and live-cell imaging
• Two cradles for other vessels
• Compatible labware: Biosensor E-Plate 96 and Biosensor E-Plate View 96
• With 96-LED brightfield module: ANSI/SLAS standard 96-well plate, 6-well plate, 35 mm and 60 mm Petri dish, and T25 flask
• Included computer running eSight Software with Basic license
• Included mouse, keyboard, monitor, power supply cables, and USB communication cable

Impedance measurement specifications

• Test signal: 22 mV rms ± 20% with maximum 5 mV DC offset at 10 kHz Ω
• Average impedance measurement speed: 2.88 s for E-Plate 96 and E-Plate View 96
• Impedance measurement accuracy: ± (1.5% + 1 Ω)
• Impedance measurement repeatability: 0.8%
• Impedance dynamic range: 10 Ω to 5 kΩ
• Electronic switch resistance: 2 to 5 Ω

Imaging specifications

• Optical imaging modes: brightfield and three fluorescence channels—blue, green, and red
• Fluorescence excitation/emission:
• Red: excitation 588–596 nm; emission 612–680 nm
• Green: excitation 473–491 nm; emission 503–561 nm
• Blue: excitation 381–404 nm; emission 430–462 nm
• Fluorescence light source: high-power LEDs
• Objective: 10x
• Imaging resolution: 1.72 µm/pixel at 10x
• 10x field of view:
• 0.85 × 0.88 mm for E-Plate View 96
• 1.68 × 1.40 mm for ANSI/SLAS standard 96-well plate
• Camera: 5.0-megapixel image
• Automated functions: autofocus and brightfield auto-exposure
• Autofocus method: image-based autofocus

Applications

• Cell characterization and quality control
• Immunotherapy and cell-killing assays
• Cell adhesion studies
• Receptor signaling research
• Live-cell imaging
• Impedance-based cell analysis
• Cellular proliferation and viability monitoring
• High-throughput cell-based assay workflows

Physical specifications

• Dimensions (W × D × H): 16.9 × 17.5 × 16.2 in. (43.1 × 44.5 × 41.4 cm)
• Weight: 60 lb (27.2 kg)
• Manufacture year: 2022

Available physical options

• 5x objective
• 20x objective
• CO2 incubator