Agilent Seahorse XFe96 Analyzer for Real-Time Cellular Metabolic Flux Analysis

The Agilent Seahorse XFe96 Analyzer is a microplate-based metabolic flux analyzer designed for high-throughput, real-time assessment of cellular bioenergetics. This instrument measures both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) simultaneously across a 96-well microplate format, making it essential for detailed analysis of mitochondrial function, glycolysis, and cellular respiration in live-cell assays.

Key features include:

• 96-well format allowing parallel analysis and increased throughput in metabolic phenotyping and drug screening workflows.
• Dual analyte optical sensor cartridge with 96 probes providing sensitive detection of metabolic changes.
• Up to four independent compound injections per well (Ports A-D) enabling dynamic modulation of cellular metabolism during assays.
• Small microchamber volume of 2 µL and assay running volume between 160–300 µL per well for efficient reagent use.
• Sample environment control with operating temperature between 16–42°C, suitable for various cell culture conditions without CO₂ or humidity control.
• Automated plate handling and precise sensor positioning ensure reproducible measurements.
• Software integration via a touchscreen computer running Wave 2.6.3 software for assay setup and data acquisition.

This system is widely adopted in biotechnology and molecular diagnostics for bioenergetic profiling, mitochondrial toxicity testing, and metabolic disease research. It supports live-cell, label-free monitoring of cellular energy metabolism, making it critical in bioprocessing, synthetic biology research, and biopharmaceutical production pipelines. This model is currently in use in research labs for applications including cellular respiration analysis, metabolic profiling, and drug response assays.

The Seahorse XFe96 analyzer comes fully serviced, tested, and calibrated to ensure operational reliability, backed by a warranty covering parts and labor. It is well-suited for academic, pharmaceutical, and clinical research environments requiring precise characterization of cellular metabolic states in a high-throughput format.