Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader

Agilent BioTek Cytation 5 CYT5MF is a multimode microplate reader configured for absorbance, fluorescence intensity, luminescence, and time-resolved fluorescence measurements. This Cytation 5 is a multimode-only configuration without imaging and uses both filter-based and monochromator-based optical pathways for flexible assay development.

• Detection modes: UV–Vis absorbance, fluorescence intensity, luminescence, and time-resolved fluorescence (TRF)
• Optical system: Quad monochromators with top and bottom fluorescence-intensity paths and a secondary TRF path
• Fluorescence intensity range: 250–700 nm
• Fluorescence bandwidth: Variable from 9–50 nm in 1 nm increments
• Fluorescence dynamic range: 7 decades
• Reading speed: 96 wells in 11 s; 384 wells in 22 s
• Absorbance range: 230–999 nm
• Absorbance bandwidth: 4 nm from 230–285 nm; 8 nm above 285 nm
• Absorbance dynamic range: 0–4.0 OD
• Absorbance resolution: 0.0001
• Wavelength accuracy: ±2 nm
• Wavelength repeatability: ±0.2 nm
• Luminescence range: 300–700 nm
• Luminescence dynamic range: >6 decades
• Incubation: Four-zone incubation up to 65 °C with condensation control
• Temperature uniformity: ±0.2 °C at 37 °C
• Plate compatibility: 6–384-well microplates, with support for Take3 microvolume plates
• Shaking: Linear, orbital, and double-orbital modes
• Dimensions: 16.4 in W × 17.5 in H × 20.2 in D
• Weight: 80 lb (36.3 kg)

The included configuration contains a custom filter cube with the following positions:

• Position 1: Excitation 485/20 nm, dichroic mirror 510 nm, emission 530/25 nm
• Position 2: Excitation 440/30 nm, dichroic mirror 510 nm, emission 530/25 nm

The system includes a power supply, communication cables, desktop computer, monitor, mouse, keyboard, and Gen5 software. It is suitable for ELISA, protein and DNA quantification, fluorescence intensity assays, ATP and reporter-gene luminescence assays, cell viability and cytotoxicity testing, enzyme activity measurements, kinetic plate assays, and multiplex biomarker detection. These capabilities support applications in biotechnology, molecular biology, cell-based research, drug discovery, and biopharmaceutical development.