Agilent BioTek Synergy H1M Multimode Microplate Reader

The Agilent BioTek Synergy H1M multimode microplate reader is designed for flexible absorbance, fluorescence intensity, luminescence, and time-resolved fluorescence analysis in life science and biotechnology laboratories. Its monochromator-based optical system supports UV-Vis measurements, fluorescence assays, kinetic studies, spectral scans, and well-area scans.

• Detection modes: UV-Vis absorbance, fluorescence intensity, luminescence, and time-resolved fluorescence
• Wavelength selection: Quadruple grating monochromator system
• Read methods: Endpoint, kinetic, spectral scanning, and well-area scanning
• Microplate formats: 6-, 12-, 24-, 48-, 96-, and 384-well plates
• Temperature control: Up to 45 °C; ±0.2 °C at 37 °C
• Shaking: Linear, orbital, and double orbital
• Included hardware: Standard power and communication cables
• Software: Gen 5 or Gen 6 software is not included and must be purchased separately
• Optional accessory: BioTek BioStack Microplate Stacker for higher-throughput workflows

Absorbance performance

• Xenon flash lamp light source
• Monochromator wavelength selection
• Wavelength range: 230–999 nm in 1 nm increments
• Bandpass: 4 nm at 230–285 nm; 8 nm above 285 nm
• Dynamic range: 0–4.0 OD
• Resolution: 0.0001 OD
• Pathlength correction: Yes
• OD accuracy: <1% at 2.0 OD; <3% at 3.0 OD
• OD repeatability: <0.5% at 2.0 OD
• Reading speed: 11 seconds for 96 wells and 22 seconds for 384 wells

Fluorescence intensity performance

• Xenon flash lamp light source
• PMT detectors
• Double grating monochromators for top and bottom reading
• Wavelength range: 250–700 nm
• Dynamic range: 5 decades
• Sensitivity with monochromators:
• Top: Fluorescein 2.5 pM, equivalent to 0.25 fmol/well in a 384-well plate
• Bottom: Fluorescein 4 pM, equivalent to 0.4 fmol/well in a 384-well plate

Luminescence performance

• Monochromator system sensitivity: 20 amol ATP, flash
• Wavelength range: 300–700 nm
• Dynamic range: >6 decades
• Full-light luminescence detection for glow and flash assays

Time-resolved fluorescence performance

• Xenon flash lamp light source
• Europium sensitivity: 1200 fM with monochromators, equivalent to 120 amol/well in a 384-well plate
• Wavelength range: 250–850 nm

The Synergy H1M is suitable for ELISA, protein quantification, DNA/RNA concentration analysis, cell viability and proliferation assays, reporter gene assays, enzyme kinetics, drug screening, and toxicity testing. It is also applicable to molecular biology, cell-based research, bioprocessing assay development, molecular diagnostics, and broader biotechnology workflows.

This pre-owned microplate reader was tested by an in-house service team for power-on operation, system initialization, control software connection, plate carrier movement and positioning, incubator performance, shaking, fluorescence, luminescence, and absorbance functionality.