Agilent BioTek Synergy H1F Filter-Based Multimode Microplate Reader

The Agilent BioTek Synergy H1F microplate reader is a filter-based multimode detection platform for fluorescence intensity, luminescence, and time-resolved fluorescence (TRF) assays. It supports endpoint, kinetic, and well-area scanning read methods for biotechnology, cell biology, enzyme activity, molecular diagnostics, and biomedical research workflows.

• Detection modes: Fluorescence intensity, luminescence, and time-resolved fluorescence
• Wavelength selection: Filter-based
• Microplate compatibility: 6-, 12-, 24-, 48-, 96-, and 384-well plates
• Temperature control: Up to 45 °C; ±0.2 °C at 37 °C
• Shaking modes: Linear, orbital, and double orbital
• Read methods: Endpoint, kinetic, and well-area scanning
• Included hardware: Power supply, communication cables, and custom filter cube
• Custom filter cube configuration:
• Position 1: Ex 485/20 nm, Dm 510 nm, Em 530/25 nm
• Position 2: Ex 440/30 nm, Dm 510 nm, Em 530/25 nm

The instrument is suitable for fluorescence intensity assays, ATP and other luminescence-based assays, TRF assays, enzyme activity testing, cell viability and cytotoxicity assays, and kinetic reaction monitoring. These capabilities support applications in cell-based screening, synthetic biology research, bioprocess development, and molecular biology laboratories.

This pre-owned unit was made in 2021, has been serviced, and was tested for power operation, system initialization, software communication, temperature control to 45 °C, all shaking modes, fluorescence detection, luminescence detection, TRF performance, and auto-exposure functionality.

Important: The computer and Gen5 software used for testing are not included and must be purchased separately.