Molecular Devices ImageXpress Micro Confocal HT.ai High-Content Laser Confocal Imaging System with Environmental Control, Automation and Water Immersion Options

The Molecular Devices ImageXpress Micro Confocal HT.ai is a high-content laser confocal imaging system designed for high-throughput fluorescence and transmitted light microscopy in biotechnology and cell biology research. It integrates a high-speed Andor Zyla sCMOS camera with a dual spinning disk confocal geometry (50 µm slit and 60 µm pinhole) enabling optical sectioning and high-resolution imaging for live or fixed cell assays.

Key technical features include:

• Laser light sources from 89 North LDI-NIR EX light engine (wavelengths at 405, 445, 470, 520, 555, 640, 730 nm) alongside a Nikon 100W halogen lamp for transmitted light imaging, supporting multiplexed fluorescence and high-contrast morphology visualization.
• Nikon water immersion objectives (20x/0.95 NA and 40x/1.15 NA) with a water immersion controller maintaining a stable water layer for improved resolution and signal fidelity in aqueous 3D tissue samples such as organoids or spheroids.
• Environmental control unit (Live Cell Instrument FC-9 Gas Mixer) regulating CO₂ (5-10%), temperature (30-40°C ±0.5°C), and humidity to support multi-day live-cell time-lapse imaging while minimizing evaporation in multiwell plates.
• Advanced automated sample handling via the Brooks PreciseFlex 400 robotic arm, equipped with multiple 20-position plate hotels, barcode reading, and precision motion control (10 µm resolution, 750 mm Z travel), enabling fully unattended, large-scale screening workflows.
• Software suite MetaXpress 6.7.2 with modules for time-series acquisition, Z-series confocal imaging, custom analysis pipelines, and transmitted light segmentation, facilitating complex image analysis crucial for molecular diagnostics and phenotypic screening in biopharmaceutical development.

The system supports slides and microplates from 1 to 1536 wells (round or flat bottom) enabling high-throughput quantitative cellular phenotyping, 3D cell model analysis, and multiplexed assays within synthetic biology, gene editing validation, and cell-based screening applications.

Installation options, warranty coverage, technical consultation, and optional remote or on-site training are provided for streamlined integration into bioprocessing and molecular biology laboratories. This platform is widely adopted in biotechnology research fields requiring precise fluorescence imaging combined with transmitted light modalities and automated high-content screening for advanced synthetic biology workflows, molecular cloning quality control, and biomanufacturing process monitoring.