Affymetrix GeneChip Human Genome U133 Plus 2.0 Array

Affymetrix U133A+2.0

The Affymetrix U133A+2.0 is a high-density DNA microarray designed for comprehensive human gene expression analysis. This array enables researchers to explore human biology and disease processes by analysing the expression levels of 18,400 transcripts and variants, including 14,500 well-characterised human genes.

Key features include:

• Comprehensive gene coverage: Provides coverage of well-substantiated genes in the transcribed human genome on a single array.
• High probe density: Comprised of more than 22,000 probe sets and 500,000 distinct oligonucleotide features, ensuring detailed and accurate gene expression profiling.
• Enhanced sensitivity: New design and reduced feature size allow the use of smaller sample volumes without compromising performance.
• Reproducibility: Multiple independent measurements for each transcript deliver high accuracy and reproducibility.

This microarray is widely utilised in various scientific fields, including molecular biology, genomics, and biotechnology. It is particularly valuable in bioprocessing and molecular diagnostics, where precise gene expression data are critical. Commonly employed in university laboratories, biomedical research centres, and biopharmaceutical companies, the Affymetrix U133A+2.0 supports a range of applications from basic research to clinical studies.

First released in the early 2000s, this array has been a cornerstone in gene expression studies. While newer technologies have emerged, the Affymetrix U133A+2.0 remains relevant due to its established reliability and extensive use in historical datasets.