Affymetrix GeneChip Human Genome U133 Array

Affymetrix U133 is a pioneering DNA microarray platform designed for comprehensive gene expression profiling in human samples. Launched in 2003, this microarray enables researchers to simultaneously analyse the expression levels of thousands of genes, providing a holistic view of the human transcriptome.

Key features include:

• Extensive genome coverage: The U133 Plus 2.0 Array interrogates nearly 50,000 RNA transcripts and variants, offering a thorough representation of the human genome.

• High probe density: Utilising over 1 million probes, with 22 independent measurements per transcript, the microarray ensures superior data quality and reliability.

• Efficient workflow: The integration with the GeneAtlas® Personal Microarray System provides a cost-effective solution for in-house whole-genome expression analysis, streamlining research processes.

• Regular updates: Affymetrix routinely updates sequence files every six months, ensuring access to the most relevant whole-genome information and the highest sequence coverage available.

This microarray is widely utilised in various scientific fields, including molecular biology, genomics, oncology, and pharmacogenomics. It is commonly employed in university laboratories, biomedical research centres, and clinical research institutions for applications such as gene expression analysis, biomarker discovery, and disease profiling.

While newer technologies have emerged, the Affymetrix U133 series remains a valuable tool in many research settings, particularly for studies requiring established and reproducible gene expression data.