Molecular Devices FlexStation 3 Multi-Mode Microplate Reader Unit 3 for Absorbance, Fluorescence, and Luminescence Detection

The Molecular Devices FlexStation 3 is a versatile benchtop multi-mode microplate reader designed for advanced absorbance, fluorescence, luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) detection. Widely adopted in molecular biology, biotechnology, and clinical diagnostics, it supports various high-throughput assay workflows essential for drug discovery, gene editing validation, and synthetic biology research.

_Key technical specifications include:_

• Detection modes: Absorbance (ABS), Luminescence (LM), Fluorescence (FL), Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP)
• Spectral range: Excitation 250–850 nm; Emission 360–850 nm (TRF, FP with slightly narrower ranges)
• Plate formats: Supports 6-, 12-, 24-, 48-, 96-, and 384-well microplates with rapid reading times (e.g., 96 well absorbance read in 18 seconds)
• Integrated fluidics system: Enables real-time reagent addition via an 8-channel pipettor for kinetic and endpoint assays, critical in bioprocessing and enzyme kinetics studies
• Temperature control: Incubator spans 2°C above ambient to 45°C, allowing incubation for live-cell assays and thermal kinetic experiments
• Shaking and trituration: Programmable shaking (0–999 seconds) facilitates homogeneous sample mixing in fluorescence and luminescence assays
• Measurement configurations: Top and bottom reading modes for fluorescence and luminescence increase assay flexibility, particularly useful in protein-protein interaction and ion channel assays
• Precision and volume: Max pipetting volume 200 µL with 2% CV at 50 µL ensures reproducible reagent delivery

This compact reader is popular among researchers in neuroscience, cell imaging, and biomanufacturing fields. It supports extensive assay customization and real-time kinetic monitoring vital for molecular diagnostics and synthetic biology workflows.

The reader comes with a trial version of SoftMax Pro v7.0 software; a software subscription is required for full functionality. Its robust design and comprehensive validation by an experienced service team ensure reliability for long-term applications in molecular cloning, drug discovery pipelines, and biotechnology labs.

_Applications include:_

• Ion channel and GPCR signaling assays
• Enzyme kinetics and cytotoxicity testing
• Protein interactions and fluorescence polarization studies
• Molecular diagnostics and biopharmaceutical development

This pre-owned unit is thoroughly tested for functionality, including power-up, software communication, fluorescence protocols, absorbance accuracy, temperature control, and mechanical operations. It is accompanied by a warranty and technical support, ensuring dependable performance in demanding research environments.

This microplate reader is essential for laboratories requiring multiplexed detection modes and real-time reagent handling, making it a critical instrument for advancing bioprocessing, gene editing validation, and synthetic biology research.