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Olympus Fluoview 1000

UsedOlympusConfocal microscopesNetherlands
Procurable

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Laser Excitation Wavelengths
405 nm, 488 nm, 543 nm, 633 nm
Laser Power
405 nm: 10 mW, 488 nm: 10 mW, 543 nm: 10 mW, 633 nm: 10 mW
Scanning Modes
1D (spot-scan), 2D (X-Y vector, X-Z, or time), 3D (X-Y-Z or X-Y-time), 4D (X-Y-Z-time)
Spectral Imaging Capabilities
Spectral detection system for high-sensitivity imaging and the ability to combine up to four fluorescent markers
Spectral Detector
Cooled GaAsP photomultiplier, 2 channels
Availability
Procurable
Lead time
Confirmed with your quote
All 18 specifications
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Specifications 18

Brands
Olympus
New/Used
Used
Laser Excitation Wavelengths
405 nm, 488 nm, 543 nm, 633 nm
Laser Power
405 nm: 10 mW, 488 nm: 10 mW, 543 nm: 10 mW, 633 nm: 10 mW
Scanning Modes
1D (spot-scan), 2D (X-Y vector, X-Z, or time), 3D (X-Y-Z or X-Y-time), 4D (X-Y-Z-time)
Spectral Imaging Capabilities
Spectral detection system for high-sensitivity imaging and the ability to combine up to four fluorescent markers
Spectral Detector
Cooled GaAsP photomultiplier, 2 channels
Spectral Method
Motorized Volume Phase Holographic transmission diffraction grating
Spectral Range
Approximately 300 nm to 850 nm
Environmental Control
Environmental chamber to maintain optimal conditions for live-cell imaging
Environmental Chamber
Included for live-cell imaging
Objectives
UPlanAPO 10X/0.40, UPlanFL 20X/0.50, UAPO/340 40X/1.50 water, UPlanFL 40X/1.3 oil, PlanApo 60X/1.4 oil, UPlanSApo 100X/1.4 oil
Transmitted Light
External halogen light source connected to microscope via fiber cables, external PMT for light collection
DIC Optics
Available for all objectives
Software
FLUOVIEW software for image capture and analysis
Power Supply
Standard laboratory power supply
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Confocal microscope with multi-laser excitation, spectral imaging, 4D scanning, and environmental control for advanced fluorescence imaging.

Olympus Fluoview 1000 is a high-performance confocal laser scanning microscope designed for advanced fluorescence imaging in life sciences research. Introduced in the early 2000s, this system enables precise, high-resolution imaging of biological specimens, making it invaluable for applications in biotechnology, neuroscience, and cell biology.

Key features include:

  • Multi-laser excitation: Equipped with four lasers (405 nm, 488 nm, 543 nm, and 633 nm), allowing versatile excitation of various fluorophores.
  • Advanced scanning modes: Supports multiple scanning configurations, including 1D (spot-scan), 2D (X-Y vector, X-Z, or time), 3D (X-Y-Z or X-Y-time), and 4D (X-Y-Z-time), facilitating comprehensive sample analysis.
  • Spectral imaging capabilities: Features a spectral detection system for high-sensitivity imaging and the ability to combine up to four fluorescent markers, enhancing multiplexing in experiments.
  • Simultaneous stimulation and imaging (SIM scanning): Enables coordinated laser stimulation and imaging, ideal for techniques like FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss in Photobleaching), and photoactivation studies.
  • Environmental control: Incorporates an environmental chamber to maintain optimal conditions for live-cell imaging, crucial for observing dynamic biological processes.

This instrument is widely utilized in university laboratories, biomedical research centres, and biotechnology companies for applications such as live-cell imaging, fluorescence resonance energy transfer (FRET) studies, and molecular diagnostics. Its versatility and precision have made it a staple in research settings focused on understanding complex biological systems.

While the Fluoview 1000 has been succeeded by newer models like the FV3000, it remains in use in many facilities due to its robust performance and comprehensive imaging capabilities.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 31 October 2026