Nikon Eclipse Ti-U Inverted Fluorescence Phase Contrast Microscope with Mechanical Stage and Fluorescence Filters

The Nikon Eclipse Ti-U is a robust inverted microscope combining fluorescence, phase contrast, and brightfield imaging capabilities, tailored for precise live-cell imaging, molecular diagnostics, and advanced biotech research applications. Designed with a manual binocular head and a mechanical XY stage, it facilitates detailed observation of live or thick specimens in Petri dishes and culture flasks, making it widely adopted in synthetic biology and molecular biology workflows.

Key technical specifications include:

• Microscope base: Nikon Eclipse Ti-U inverted base with dual camera ports and illumination arm.
• Illumination:
• Fluorescence: Nikon 100 W full spectrum HBO lamp with power supply, mechanical 6-position filter cube turret housing three brand new filter sets (e.g., DAPI, FITC, Texas Red, CY3) compatible with dyes like AlexaFluor and Rhodamine.
• Brightfield: Nikon 100 W halogen light source with power supply and illumination tower.
• Objectives: Mechanical 6-position turret with CFI Plan Fluor PhL and Ph1 objectives (4x/0.13, 10x/0.30, 20x/0.45) enabling magnifications from 40x to 1000x with 10x eyepieces.
• Condenser: Mechanical condenser with 0.52 lens and phase rings for PhL and Ph1.
• Camera: Bestscope 8.3 MP color CMOS camera with 1x C-mount adapter, complemented by Micromanager and ImageJ software for image acquisition, calibration, measurement, and analysis.
• Stage: Smooth mechanical XY stage with options for motorization and joystick control to enhance bioprocessing workflows and multi-sample scanning.

Optical performance is verified by field distortion and fluorescence intensity measurements at the specimen plane. Preventive maintenance ensures reliable illumination and filter performance critical for biotech and clinical diagnostics. The inverted design and phase contrast capability provide enhanced contrast for unstained, transparent specimens, widely used in live-cell imaging and pharmaceutical research.

Customizable upgrade options enable advanced fluorescence with LED, Xenon, or metal halide light sources and additional filter sets for extended spectral compatibility. High-end CCD, sCMOS, or EMCCD camera modules are available for enhanced imaging quality in biomanufacturing and molecular cloning workflows. Motorized stages and automation support complex synthetic biology and biomedical engineering experiments.

This microscope is essential for biotechnology professionals conducting gene editing experiments, molecular diagnostics, or biopharmaceutical production pipeline research due to its versatile imaging modalities and configuration flexibility.

Included services: comprehensive servicing, warranty (180 days USA, 60 days international; optional extended warranty), remote training sessions, and software overview. Shipping utilizes ISPM15-certified wooden crates or secure parcel packing, ensuring safe delivery and operational integrity of sensitive optical equipment.

Ideal for applications in:

• Fluorescence and phase contrast microscopy for biotechnology and molecular biology research
• Live-cell imaging and intracellular studies in biomolecular engineering
• Pharmacological and drug screening studies
• Cellular structure and function analysis in physiology and clinical diagnostics
• Educational and research training in universities and institutes

The Nikon Eclipse Ti-U is a pre-owned, fully serviced instrument recognized for adaptability, optical precision, and compatibility with demanding fluorescence and phase contrast imaging applications critical to advanced biomedical and biotechnological research environments.