Leica TCS SP2 AOBS

The Leica SP2 AOBS is a laser scanning confocal microscope renowned for its high-resolution imaging capabilities, making it indispensable in various scientific research fields. This instrument excels in capturing detailed images of both fixed and live specimens, facilitating advanced studies in cellular and molecular biology.

Key features include:

• Acousto-Optical Beam Splitter (AOBS): Allows flexible and rapid selection of excitation wavelengths without the need for manual filter changes.
• Multiple Laser Lines: Equipped with lasers at 405 nm, 458 nm, 476 nm, 488 nm, 496 nm, 514 nm, 543 nm, 594 nm, and 633 nm, enabling excitation of a wide range of fluorophores.
• Spectral Imaging: Facilitates precise separation and analysis of overlapping fluorescence signals.
• Time-Lapse Imaging: Captures dynamic processes over time, essential for live-cell studies.
• 3D Z-Stacks: Constructs three-dimensional representations of specimens by compiling multiple focal planes.
• Advanced Techniques: Supports Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Resonance Energy Transfer (FRET), and Fluorescence Loss in Photobleaching (FLIP) experiments.

This confocal microscope is widely applied in fields such as neuroscience, molecular biology, biotechnology, and biomedical engineering. Its versatility makes it suitable for various applications, including live-cell imaging, fluorescence analysis, and bioprocessing studies.

Commonly found in university laboratories, biomedical research centres, and clinical environments, the Leica SP2 AOBS has been a cornerstone in scientific imaging since its release in the early 2000s. While newer models have emerged, this system remains in use due to its reliability and comprehensive feature set.