Leica Microsystems TCS SP8 AOBS

The Leica AOBS (Acousto-Optical Beam Splitter) is an advanced beam-splitting technology integrated into Leica's confocal microscopy systems, such as the TCS SP2 and SP5 models. Introduced in the early 2000s, this innovation enhances fluorescence imaging by offering unparalleled flexibility and efficiency in separating excitation and emission light.

Key features:

• High transparency and narrow excitation bands: Increases cell viability and reduces photobleaching, crucial for live-cell imaging.
• Freely programmable beam splitting: Adapts to challenging multicolour experiments and accommodates new laser lines without the need for physical filter changes.
• Fast line sequential imaging: Enables rapid imaging of living cells with spectrally close fluorophores, essential for dynamic studies.
• Enhanced signal detection: Provides steep edges and narrow bandwidths, allowing the collection of more emission light compared to traditional dichroic mirrors.

The Leica AOBS technology is widely utilised in various scientific fields, including:

• Cell biology
• Neuroscience
• Molecular biology
• Biotechnology

It is commonly employed in institutions such as:

• University laboratories
• Biomedical research centres
• Clinical research facilities

By offering a flexible and efficient solution for beam splitting, the Leica AOBS has become a critical component in confocal microscopy, supporting a wide range of applications from basic research to advanced biotechnological studies.