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Life Technologies pcDNA3.1(-)

UsedLife TechnologiesGenome editing and molecular toolsNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Plasmid Length
5428 bp
Availability
Procurable
Lead time
Confirmed with your quote
All 5 specifications
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Specifications 5

Brands
Life Technologies
New/Used
Used
Plasmid Length
5428 bp
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Plasmid vector for high-level, constitutive recombinant protein expression in mammalian cells with CMV promoter and selectable markers.

pcDNA™3.1(-) Mammalian Expression Vector is a versatile plasmid designed for high-level, constitutive expression of recombinant proteins in mammalian cell lines.

Key features include:

  • CMV promoter: Ensures robust transcription initiation for high protein yields.
  • Multiple cloning site (MCS): Located in the reverse (-) orientation, facilitating flexible gene insertion.
  • Geneticin™ (G-418) resistance gene: Allows for efficient selection of stable transfectants in mammalian cells.
  • Ampicillin resistance gene: Enables bacterial selection and propagation.
  • SV40 origin of replication: Supports episomal replication in cells expressing the large T antigen, such as COS-1 and COS-7.
  • Bovine Growth Hormone (BGH) polyadenylation signal: Enhances mRNA stability, leading to increased protein expression.

This vector is widely utilised in molecular biology, biotechnology, and biomedical research for applications such as gene expression studies, protein production, and functional analyses. It is commonly employed in university laboratories, biotechnology companies, and pharmaceutical research centres. Since its introduction, pcDNA™3.1(-) has remained a staple in molecular cloning and gene expression workflows, underscoring its enduring relevance in the field.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Can't find the exact configuration?

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Last updated 19 October 2026