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Procurable · sourced on request

Invitrogen pCR2.1-TOPO

UsedInvitrogenGenome editing and molecular toolsNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Vector Length
3931 bp
Copy Number
High copy number
Antibiotic Resistance
Ampicillin
Selection Marker
Neomycin/G418 (Geneticin)
Promoter
T7
Availability
Procurable
Lead time
Confirmed with your quote
All 11 specifications
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Specifications 11

Brands
Invitrogen
New/Used
Used
Vector Length
3931 bp
Copy Number
High copy number
Antibiotic Resistance
Ampicillin
Selection Marker
Neomycin/G418 (Geneticin)
Promoter
T7
Primer Sites
M13 forward and reverse
Restriction Sites
EcoR I
Availability
Procurable
Lead time
Confirmed with your quote

Product description

High-copy plasmid vector for direct cloning of Taq-amplified PCR products with dual antibiotic selection.

The Invitrogen pCR2.1-TOPO vector is a high-copy number plasmid designed for rapid and efficient cloning of PCR products, particularly those amplified using Taq DNA polymerase. This vector facilitates the direct insertion of PCR products into a plasmid without the need for post-PCR modifications, streamlining the cloning process.

Key features:

  • 3'-T overhangs: Allows direct ligation of Taq-amplified PCR products.
  • T7 promoter: Enables in vitro RNA transcription and sequencing.
  • M13 forward and reverse primer sites: Facilitates sequencing and PCR screening.
  • EcoR I sites: Flank the insertion site for easy excision of inserts.
  • Dual antibiotic resistance genes: Provides selection options with ampicillin and kanamycin.
  • Blue/white colony screening: Simplifies identification of recombinant clones.

This vector is widely utilised in molecular biology and biotechnology research for applications such as gene cloning, sequencing, and in vitro transcription. It is commonly employed in university laboratories, biomedical research centres, and biotechnology companies. Since its introduction, the pCR2.1-TOPO vector has remained a valuable tool in molecular cloning workflows.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 5 November 2026