Invitrogen pCR2.1

Invitrogen pCR2.1 is a versatile cloning vector designed to facilitate the efficient insertion of Taq DNA polymerase-amplified PCR products into plasmid vectors. This system streamlines molecular cloning workflows, making it indispensable in various biotechnology applications.

Key features include:

• Rapid ligation: Achieves ligation in just 15 minutes at room temperature, enhancing laboratory efficiency.
• High efficiency: Utilises blue/white screening, yielding over 80% recombinants with the correct insert.
• Dual antibiotic resistance: Offers both kanamycin and ampicillin resistance, providing flexibility in antibiotic selection.
• User-friendly design: Eliminates the need for enzymatic modifications of PCR products and primers with restriction sites.
• Versatile polylinker: Features flanking EcoR I sites for easy excision of inserts, facilitating downstream applications.

This vector is widely employed in molecular biology, genetic engineering, and synthetic biology research. It is particularly valuable in biotechnology sectors, including gene editing, molecular diagnostics, and biomanufacturing.

Commonly utilised in university laboratories, biomedical research centres, and biotechnology companies, the pCR2.1 vector has been a staple in molecular cloning since its introduction. Its enduring relevance underscores its reliability and effectiveness in advancing genetic research and biotechnological innovations.