Promega psiCHECK-2

The Promega psiCHECK-2 Vector is a specialised plasmid designed to optimise RNA interference (RNAi) assays by enabling the quantitative monitoring of gene expression changes. This vector incorporates Renilla luciferase as the primary reporter gene, with a multiple cloning site downstream of its stop codon for inserting the gene of interest. Upon initiation of RNAi, the fusion mRNA undergoes degradation, leading to a measurable decrease in Renilla luciferase activity, thereby providing a sensitive indicator of RNAi efficacy.

Key features include:

• Dual-reporter system: In addition to Renilla luciferase, the vector contains firefly luciferase under a separate promoter, allowing for normalisation of Renilla activity and enhancing result robustness.

• Endpoint lytic assays: Specifically designed for endpoint measurements, facilitating straightforward and reproducible data collection.

• High sensitivity: Offers more convenient and rapid quantitation with higher sensitivity compared to other fusion approaches, such as GFP or flag-tags.

The psiCHECK-2 Vector is widely utilised in molecular biology, genetics, and biotechnology research, particularly in studies involving gene silencing, microRNA function, and RNAi mechanism exploration. It is commonly employed in university laboratories, biomedical research centres, and biotechnology companies. Since its release, the psiCHECK-2 Vector has remained a relevant and valuable tool in the field, continuing to support advancements in gene expression analysis and RNAi research.