Addgene pLKO.1-puro

psPAX2 is a second-generation lentiviral packaging plasmid designed to facilitate the production of high-titre lentiviral particles. This plasmid is integral to the generation of lentiviral vectors, which are widely used for stable gene delivery in mammalian cells.

Key features include:

• Compatibility: Works seamlessly with both second and third-generation lentiviral vectors, as well as envelope-expressing plasmids like pMD2.G.
• High Titre Production: Yields higher viral titres compared to other packaging plasmids, such as pCMV-dR8.2 dvpr.
• Essential Components: Encodes critical viral proteins including Gag, Pol, Rev, and Tat, necessary for efficient viral particle assembly.

This plasmid is extensively utilised in:

• Molecular Biology: For gene expression studies and functional genomics.
• Biotechnology: In applications like gene editing and synthetic biology.
• Biomedical Research: For developing gene therapies and studying disease mechanisms.

Commonly employed in:

• University Laboratories: For academic research and teaching purposes.
• Biotechnology Companies: In the development of gene-based products and therapies.
• Clinical Research Centres: For translational research and clinical trials.

Since its deposition by the Trono lab, psPAX2 has been a cornerstone in lentiviral vector production, maintaining its relevance in contemporary research and biotechnological applications.