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Biotek FLx800 Fluorescence Microplate Reader for DNA, Protein, and Cell-Based Quantification

UsedBioTekMicroplate readersUnited States

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Fluorescence Intensity Wavelength Range
300-650 nm Ex 340-700 nm EM
Dynamic Range
5 Decades
Sensitivity Top
5 pM Fluorescein typical
Wavelength Selection
Deep blocking filters
Light Source
Tungsten halogen, 1000 hour life
All 13 specifications
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Specifications 13

Brands
BioTek
New/Used
Used
Fluorescence Intensity Wavelength Range
300-650 nm Ex 340-700 nm EM
Dynamic Range
5 Decades
Sensitivity Top
5 pM Fluorescein typical
Wavelength Selection
Deep blocking filters
Light Source
Tungsten halogen, 1000 hour life
Luminescence Wavelength Range
300-700 nm
Sensitivity
100 amol ATP typical (flash)
Microplate Types
6- to 384- well
Dimensions
17x15x8”
Weight
30 lbs
Power
115V 50/60Hz

Product description

High-sensitivity Biotek FLx800 fluorescence microplate reader with 300-700 nm range, suitable for DNA and protein quantification.

The Biotek FLx800 Fluorescence Microplate Reader is a precision instrument designed for advanced fluorescence and luminescence detection in biotechnology and molecular diagnostics applications. Featuring a wavelength excitation range of 300–650 nm and an emission range of 340–700 nm, this reader achieves a fluorescence intensity sensitivity of 5 pM fluorescein and luminescence sensitivity down to 100 amol ATP, supporting a wide 5-decade dynamic range.

Key technical specifications include:

  • Light Source: Tungsten halogen lamp with 1000-hour lifespan, delivering stable excitation illumination
  • Detection: Single-channel PMT housed in a light-tight compartment for low noise and enhanced measurement sensitivity
  • Plate Formats: Compatible with microplates ranging from 6 to 384 wells, enabling diverse assay throughput
  • Features: Stationary optics, deep blocking filters for precise wavelength selection, and robust onboard software enabling custom assay setup, data manipulation, and detailed kinetic analysis
  • Automation: The FLx800TB variant supports automated top and bottom reading probes, enhancing throughput and assay versatility
  • Dimensions: Compact footprint at 17 x 15 x 8 inches and weighing 30 lbs, suitable for benchtop deployment in molecular biology and synthetic biology labs
  • Power Requirements: Operates on 115V, 50/60Hz power supply

This instrument is widely adopted in applications such as DNA quantification, Förster Resonance Energy Transfer (FRET), gene expression analysis, protein quantification, kinetic studies, and cell-based assays. Its high-sensitivity fluorescence and luminescence detection capabilities make it critical in bioprocessing and molecular cloning workflows within biotechnology and biomedical research environments.

The included Gen5 software facilitates precise assay control, data graphing, and exporting, optimizing workflows in gene editing research, molecular diagnostics, and biomanufacturing pipelines. With storage capacity for up to 75 assays, customizable excitation/emission filters, and adaptable plate geometry, the FLx800 enables reproducible and scalable measurement solutions for advanced biotechnological research.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 19 October 2026