Agilent BioTek Cytation 5 CYT5MF Multimode Microplate Reader with UV-Vis, Fluorescence, TRF, Luminescence Detection

The Agilent BioTek Cytation 5 CYT5MF is a sophisticated *multimode microplate reader* engineered for advanced biotechnology and molecular diagnostics research. It integrates filter- and monochromator-based optical systems enabling a versatile detection portfolio including UV-Vis absorbance (230–999 nm), fluorescence intensity (250–700 nm), time-resolved fluorescence (TRF), and luminescence (300–700 nm).

Key features include:

• Dual-readout flexibility with quad monochromators for absorption and fluorescence, supporting both top and bottom detection.
• Variable bandwidth control (9–50 nm for fluorescence intensity), offering fine spectral resolution essential for multiplex biomarker detection and gene editing assay workflows.
• High sensitivity fluorescence dynamic range (7 decades) and luminescence detection (>6 decades), critical for quantitative protein/DNA quantification, ELISA assays, and bioprocessing analytics.
• Rapid plate reading capability: 96 wells in 11 seconds and 384 wells in 22 seconds, suited for high-throughput screening and kinetic studies in synthetic biology and biomanufacturing applications.
• Environmental control with a 4-zone incubation system (up to 65 °C) featuring condensation control and precise temperature uniformity (±0.2 °C at 37 °C), supporting live-cell imaging and cell viability assays.
• Compatible with microplates from 6 to 384 wells, including Take3 microvolume plates, enabling flexibility in sample format and throughput requirements.
• Integrated shaking modes (linear, orbital, double orbital) to ensure homogenous sample mixing for enzyme kinetics and cytotoxicity assays.
• Delivered with Gen5 software on a dedicated desktop computer for instrument control, data acquisition, and advanced fluorescence analysis workflows.

This system is widely adopted among molecular biology researchers and clinical diagnostic labs requiring robust multimode detection without imaging capability. It is critical for biopharmaceutical production pipelines, molecular cloning workflows, and fluorescence-based live-cell viability assays. The Cytation 5 CYT5MF's precise optical performance and environmental control make it suitable for demanding biotechnological applications including molecular diagnostics, synthetic biology research, and bioprocess development.

Comprehensive functional testing by in-house service experts ensures dependable power operation, software communication, accurate optical measurements, reliable incubation, and shaking performance. The instrument ships with warranty coverage, remote setup, and training, underscoring its readiness for integration into biotechnology and life science laboratory environments.

Keywords incorporated: multimode microplate reader, UV-Vis absorbance, fluorescence intensity, time-resolved fluorescence, luminescence detection, 4-zone incubation, molecular diagnostics, synthetic biology, bioprocessing, gene editing workflows, ELISA assays, live-cell imaging, biomarker detection, quantitative protein/DNA assays, biomanufacturing.