Agilent BioTek Synergy H1F Filter-Based Multimode Microplate Reader

The Agilent BioTek Synergy H1F is a filter-based multimode microplate reader designed for precise detection across fluorescence intensity, luminescence, and time-resolved fluorescence (TRF) assays. It supports microplate formats ranging from 6- to 384-wells, accommodating diverse bioassay needs critical in biotechnology, molecular diagnostics, and bioprocessing workflows.

Key technical features include:

• Wavelength selection via interchangeable optical filters, enabling customizable excitation and emission detection (e.g., filter cubes with Ex 485/20 nm, Dm 510 nm, Em 530/25 nm)
• Detection modes: fluorescence intensity, luminescence (e.g., ATP assays), and TRF for enhanced sensitivity in time-resolved applications
• Read methods: endpoint, kinetic measurements for real-time reaction monitoring, and well area scanning for spatial signal distribution analysis
• Temperature control: incubation capability from ambient up to 45 °C, with ±0.2 °C stability at 37 °C, essential for live-cell assays and enzymatic reactions
• Shaking options: linear, orbital, and double orbital shaking to optimize reagent mixing and assay homogeneity
• Compatible microplate types: 6-, 12-, 24-, 48-, 96-, and 384-well plates, facilitating high-throughput screening and multiplexed assays

This microplate reader is widely adopted in biotechnology research, including applications such as enzyme activity testing, cell viability assays, cytotoxicity screening, and kinetic reaction analysis. Its multimode detection system supports advanced fluorescence assays critical for synthetic biology and molecular cloning workflows and is instrumental in biopharmaceutical production pipelines requiring robust molecular diagnostics.

The instrument tested fully functional with confirmed performance in fluorescence, luminescence, and TRF modes using validated test plates. It includes auto-exposure capability and comes with power and communication cables plus custom filter cubes for flexible excitation/emission configurations.

This platform is engineered for reliable, sensitive detection in biomedical engineering, live-cell imaging, and fluorescence analysis, making it essential equipment for research laboratories focused on molecular biology and bioprocess development.

Please note the Gen5 control software and computer are not included and must be purchased separately.

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