Teledyne ISCO CombiFlash EZ Prep UV/ELSD Preparative Flash Chromatograph System with Dual UV and ELSD Detection

The Teledyne ISCO CombiFlash EZ Prep UV/ELSD Preparative Flash Chromatograph System integrates flash purification and preparative-scale HPLC to deliver versatile compound separation and purification for biotechnology, pharmaceutical, and chemical research applications. This benchtop preparative chromatography system offers a dual-mode design enabling seamless switching between high-pressure prep HPLC and lower pressure flash chromatography, critical in bioprocessing and synthetic biology workflows.

Key Technical Features:

• Flow rate range: 5–200 mL/min with ±5% accuracy, supporting efficient preparative workflows from milligram to tens of grams scale.
• Pressure capability: Up to 3500 psi (240 bar) for high-pressure mode and 200 psi (13.8 bar) for flash operation, enabling purification of complex synthetic organic compounds and biochemical extracts.
• Detection: Dual detectors include variable wavelength UV detector (200–400 nm in 5 nm increments) and built-in ELSD (Evaporative Light Scattering Detector), allowing monitoring of analytes with and without UV chromophores—essential for molecular diagnostics and natural product isolation.
• Gradient capabilities: Binary gradient mixing using any two of four solvents with programmable linear, step, and isocratic gradients, ±3% gradient accuracy for precise separations.
• Sample handling: Automated self-cleaning Flash injection valve and Prep HPLC loop injection, enhancing reproducibility in purification processes for gene editing and biopharmaceutical production pipelines.
• Temperature control range: Ambient operation between 20°C and 40°C with ELSD drift tube at 30–90°C and spray chamber at 10–60°C, suitable for sensitive biomolecule purification.
• System dimensions: Compact footprint at 43 x 43 x 67 cm (17 x 17 x 26.5 in), weighing 43.6 kg (96.1 lbs), ideal for space-constrained laboratories.
• Power requirements: 100–240 VAC, 50/60 Hz for global compatibility.

This system is widely adopted in biotechnology research for applications including preparative-scale purification of synthetic compounds, isolation of reaction products in medicinal chemistry, and preparatory clean-up of biologically derived intermediates. Its combination of high-pressure preparative HPLC and flash chromatography modes makes it critical in biopharmaceutical production pipelines and molecular cloning workflows.

Included accessories: Intake hoses with weighted solvent filters, waste hoses, column bypass tube, power cable. Columns and glassware are sold separately, allowing customization of purification setups.

Operational Verification: The instrument undergoes rigorous testing including power/initiation checks, touchscreen calibration, RFID-based column/rack verification, pump priming, gradient and flow rate accuracy validation, and UV/ELSD detector functionality confirmed by caffeine test injections. This ensures reliable performance for complex purification tasks required in synthetic biology and molecular diagnostics.

Applications:

• Purification of synthetic organic molecules in pharmaceutical R&D
• Isolation and purification of biologically active natural products
• Preparative separation in molecular biology and gene editing workflows
• Clean-up of intermediates in chemical synthesis for biomanufacturing
• Preparative compound purification in clinical diagnostics and bioengineering

This Teledyne ISCO CombiFlash EZ Prep UV/ELSD System provides a robust, versatile platform optimized for preparative flash chromatography and high-pressure liquid chromatography, delivering precision and efficiency essential for advanced biotech and chemical research laboratories worldwide.