Teledyne ISCO ACCQPrep HP150 UV-Vis Preparative Liquid Chromatography System with AS 2x2 Autosampler

The Teledyne ISCO ACCQPrep HP150 UV-Vis Preparative Liquid Chromatography (LC) System is a fully integrated platform engineered for automated prep-scale purification essential in biotechnology workflows such as peptide purification, natural product isolation, and molecular diagnostics. This system features a maximum flow rate of 150 mL/min with flow rate accuracy of ±2% and a pressure limit of up to 6000 psi (414 bar), suitable for high-throughput compound purification and reaction cleanup.

Key components include:

• UV-Vis detector with wavelength range 200 to 800 nm adjustable in ±5 nm increments for sensitive absorbance tracking.
• AS 2x2 Autosampler module enabling automated, queue-based sample injection from multiposition racks, improving reproducibility and throughput for bioprocessing applications.
• ACCQPrep CSV 4 Column Selector Valve Module permitting up to four-column switching for method flexibility.
• ACCQPrep SSV 2x3 Solvent Selector Valve Module supporting up to six solvents with programmable gradients (linear, step, isocratic), ensuring precise solvent mixing critical in fractionation and biomanufacturing workflows.
• AutoInjector (AI 5) for precise, programmable sample introduction synchronized with the system’s workflow.
• Internal and optional external fraction collection integrated with autosampler control for streamlined purification workflows in synthetic biology and molecular cloning pipelines.

The system’s high-pressure dual-piston reciprocating pumps ensure stable gradient formation with gradient linearity within ±1%, reducing baseline instability and gradient drift. Compact dimensions and adaptable ambient operation temperature between 20–40 °C support installation in diverse lab environments, including clinical diagnostics labs focused on molecular purification.

This preparative LC system is widely adopted in biotechnological research settings requiring robust, unattended fraction collection and chromatographic separation with accurate retention time verification. Comprehensive in-house testing covers flow and pressure stability, autosampler performance, valve operation, and UV-Vis lamp functionality, ensuring instrument reliability from installation through after-sales support.

Benefits to biotechnology researchers include:

• Automated, reproducible sample handling for high recovery and low carryover in complex molecule purification.
• Flexible solvent and column selection facilitating method development in peptide synthesis and natural product isolation.
• Accurate gradient control vital for sensitive fractionation in gene editing and molecular diagnostics workflows.

The instrument arrives fully serviced, with tested autosampler rack compatibility, waste management accessories, and warranty backing, making it a critical tool for bioanalytical labs focusing on bioprocessing and clinical R&D. Optional remote or onsite installation and training are available, enhancing integration into biomanufacturing quality control and synthetic biology research pipelines.

Technical Specifications:

• Max flow rate: 150 mL/min
• Flow rate accuracy: ±2%
• Pressure limit: up to 6000 psi (414 bar)
• UV-Vis detection range: 200–800 nm, ±5 nm steps
• Solvents: 2x3 solvent selector valve (6 total)
• Gradient programmability: linear, step, isocratic
• Gradient linearity: ±1%
• Dimensions (LC system): 27.5 x 14.0 x 20.0 in (69.9 x 35.6 x 50.8 cm)
• Weight (LC system): 93.2 lbs (42.3 kg)
• Dimensions (Autosampler AS 2x2): 18.9 x 14.0 x 19.3 in (48.0 x 35.6 x 49.0 cm)
• Weight (Autosampler): 27.3 lbs (12.4 kg)
• Power: 100-240 VAC, 50/60 Hz

Ideal Applications:

• Preparative compound purification and reaction cleanup
• Peptide fractionation and purification critical in synthetic biology and molecular cloning
• Natural product isolation for bioprospecting and biotechnology R&D
• Bioprocessing and clinical molecular diagnostics requiring robust, high-throughput preparative chromatography

This system represents a reliable, precision-controlled preparative LC solution, essential for bioanalytical laboratories requiring stable UV-Vis detection coupled with automated sample handling and solvent/column flexibility to support advanced biomanufacturing and molecular biology research.