Molecular Devices ImageXpress Micro 4 Automated High Content Imaging System with sCMOS Camera

The Molecular Devices ImageXpress Micro 4 High Content Imaging System is an advanced automated microscopy platform designed for high-throughput, quantitative imaging of multiwell plates up to 1536 wells and microscope slides. Featuring a high-sensitivity Andor Zyla sCMOS camera, this system delivers a large 10x field of view (1.97 mm²) with rapid image acquisition speeds exceeding 200,000 wells per day, ideal for biotechnological applications such as molecular diagnostics, phenotypic screening, and bioprocessing research.

Key system components include:

• Five-position filter changer with emission filters for prominent fluorophores: DAPI, FITC, TRITC, and CY5 enabling multicolor fluorescence imaging critical for gene expression studies and protein localization assays.
• Four-position Nikon objective turret housing Plan APO objectives at 4x (0.20 NA), 10x (0.45 NA), 20x (0.75 NA), and 40x (0.95 NA), facilitating versatile magnification and resolution for applications ranging from cell viability to 3D spheroid analysis.
• Laser and image-based autofocus, optimizing z-plane focus precision for robust quantitative imaging workflows including live-cell imaging and synthetic biology experiments.
• Compatibility with robotics and lab automation systems ensures seamless integration into biomanufacturing pipelines and synthetic biology screening platforms.
• The system supports widefield fluorescence and brightfield imaging modes, enabling complex phenotypic assays and transmitted light segmentation analysis.

The included MetaXpress 6.7 software suite with PowerCore license offers comprehensive acquisition and analysis modules:

• Time-series and Z-series acquisition with integrated wizards for streamlined experimental setup.
• Customized analysis through the Custom Module Editor enables tailored image processing workflows essential for molecular cloning and molecular biology research.
• Advanced 3D acquisition tools support Z-stack capture and 2D projections, aiding 3D cell culture and organoid imaging studies.
• Kinetic measurements and curve fitting assist in biophysical assays and pharmacological profiling.

This system is widely used in cell migration, wound healing assays, phenotypic compound screening, and protein expression quantification. Its high-throughput capabilities and compatibility with fluorescent and luminescent assay kits make it essential for biotechnology fields including synthetic biology, molecular diagnostics, and biopharmaceutical production.

Comprehensive in-house testing confirms full functionality with verified optical alignment, filter performance, laser autofocus reliability, camera clarity, and stage precision ensuring image quality uniformity and data reproducibility. The system ships with approved warranties and offers remote setup and training, making it a reliable choice for research institutes, clinical labs, and biotech industry professionals seeking a versatile and robust automated imaging solution.

Primary Keywords: high content imaging system, automated fluorescence microscope, sCMOS camera imaging Long-tail Keywords: high-throughput multiwell plate imaging system, multicolor fluorescence microscopy for molecular diagnostics, automated imaging for bioprocessing workflows, laser autofocus high content imaging, quantitative phenotypic screening microscope, widefield fluorescence microscope for cell viability assays.