Bio-Rad Bio-Plex 200 Suspension Array Analyzer with HTF High-Throughput Flow Cytometry System

The Bio-Rad Bio-Plex 200 w/ HTF Suspension Array System Analyzer is a robust, compact bead-based flow cytometry platform enabling multiplex quantitative protein analysis. It supports mid-throughput applications critical for *molecular diagnostics*, *bioprocessing*, and *biotechnology research*. Designed for use with 96-well microplates (maximum thickness 0.75 in), it integrates a high-throughput HTF sheath delivery system for increased sample processing speed and reproducibility.

Key Features:

• Dual-laser configuration: 532 nm reporter laser and 635 nm classification laser for precise bead discrimination and analyte detection.
• Compatible beads: MagPlexC, MicroPlex, LumAvidin, xTAG bead sets enabling flexible assay formats.
• Multiplex capacity: simultaneous detection of up to 100 analytes per sample, essential for comprehensive cytokine profiling, synthetic biology assays, and gene editing workflows.
• Sample volume: 25–80 µL with a sheath flow rate of 90 µL/sec and sample injection rate of 60 µL/min.
• Read time: approximately 40 minutes per 96-well plate, supporting efficient high-throughput biopharmaceutical screening and molecular cloning research.
• Dynamic range: 3.5 logs for wide concentration measurement, enabling accurate quantification in vaccine development and infectious disease diagnostics.

System Components Include:

• XYP microplate stage
• HTF Sheath Delivery System for automated fluid handling
• Control computer with Bio-Plex Manager software v6.2 and USB license dongle
• Complete fluidics set with waste and sheath fluid hoses compatible with bulk containers
• All required communication and power cables

Performance Validation & Service:

The instrument undergoes exhaustive fluidics system decontamination, laser calibration, and performance verification, ensuring minimal electronic noise and consistent coefficient of variation (CV) for bead standards across all detection channels. These features are critical for maintaining signal fidelity in sensitive research domains like cell imaging and biomanufacturing. The device also features automatic quality control and system checks for bead type and analyte attachment status.

Applications:

• Multiplex cytokine profiling for autoimmune and cancer biomarker discovery
• Vaccine efficacy and pharmacokinetics studies
• Infectious disease diagnostics
• Synthetic biology and molecular cloning workflows requiring multiplex protein quantitation

Warranty & Support:

• 120 days continental US warranty and 60 days international warranty
• Remote instrument setup and training available
• Proven reliability supported by dedicated after-sales technical service and extended warranty options

This analyzer is widely adopted in clinical diagnostics and biopharmaceutical production pipelines, making it an essential tool for laboratories focused on advanced biomarker discovery and multiplex protein analysis in biotechnology and biomedical engineering.