BD Biosciences Cytofix/Cytoperm

BD Biosciences Cytofix/Cytoperm™ is a specialised reagent system designed for the simultaneous fixation and permeabilisation of cells, facilitating the intracellular staining of cytokines and other proteins for flow cytometry analysis. This solution preserves cellular morphology and antigenicity, enabling accurate detection of intracellular targets.

Key features include:

• Simultaneous fixation and permeabilisation: Streamlines sample preparation by combining both steps, reducing processing time.
• Preservation of cellular integrity: Maintains light-scattering properties and fluorescence intensities, ensuring reliable flow cytometry results.
• Compatibility with various cell types: Effective for human and rodent lymphoid and haematopoietic cells.
• Stability for extended analysis: Fixed cells can be stored for 24–72 hours post-staining without significant signal loss.

This reagent is widely utilised in:

• Immunology: For analysing intracellular cytokine production.
• Cell biology: To study intracellular signalling pathways.
• Biotechnology: Essential in molecular diagnostics and bioprocessing applications.

Commonly employed in:

• University laboratories: For academic research and teaching.
• Biomedical research centres: Conducting advanced cellular analyses.
• Clinical environments: For diagnostic and therapeutic research.

First introduced prior to 2022, BD Cytofix/Cytoperm™ remains a critical tool in flow cytometry workflows, particularly in biotechnology and biomedical research.