Bruker IsoPlexis ISOLIGHT-1000-1 Single Cell Secreted Protein Analysis System for High-Throughput Secretome Profiling

The Bruker IsoPlexis ISOLIGHT-1000-1 is an advanced single-cell secreted protein analysis system designed for precise and sensitive multiplex cytokine and chemokine profiling. Utilizing innovative microfluidic technology, this instrument enables comprehensive secretome characterization at single-cell resolution, supporting research in immunology, immuno-oncology, infectious diseases, and stem cell biology.

Key technical specifications include:

• High-throughput capacity: analyzes over 1,250 isolated cells per IsoCode disposable chip, with up to 8 chips per run enabling >10,000 isolated cells and a maximum of 360,000 data points per experiment.
• Multiplex panel detection: simultaneous profiling of up to 36 analytes per single cell.
• Operational efficiency: hands-on time under 3 minutes per sample (excluding cell preparation) and run times of less than 24 hours from sample loading to results.
• Integrated environmental control: onboard incubator maintains 37 ± 2°C temperature and 5 ± 1% CO₂ for optimal cell viability during assays.
• Optical capabilities: laser wavelengths at 405 nm, 437 nm, and 638 nm enable sensitive fluorescence detection for secreted proteins.
• Compact footprint: Dimensions 28.5" x 28" x 30.5" (72.5 x 71 x 77.5 cm), weight approx. 114 kg; supports 100-240 VAC power input.

The system is supported by the IsoSpeak analysis software (version 2.9.0) running on an included laptop, enabling real-time data acquisition and advanced immune function analysis. It employs disposable consumables, including IsoCode chips with barcode tracking and reagent cartridges, ensuring experimental reproducibility and contamination control.

Widely adopted in molecular diagnostics, bioprocessing, and biopharmaceutical production pipelines, the ISOLIGHT-1000-1 facilitates critical insights for cancer immunotherapy development, gene editing research, synthetic biology applications, and clinical diagnostics. Its precision multiplex protein analysis is essential for single-cell studies involving immune profiling, cellular secretomics, and biomanufacturing quality control.

This robust instrument is fully tested, warranted, and available with technical sales consultation. It is ideal for biotechnology laboratories requiring sensitive, high-throughput single-cell secreted protein measurement, accelerating translational research and bioengineering workflows.