Revvity Perkin Elmer Operetta CLS Automated High Content Cellular Imaging System

The Revvity Perkin Elmer Operetta CLS High Content Analysis System is an advanced benchtop platform designed for automated cellular imaging applications critical in molecular diagnostics, bioprocessing, and biopharmaceutical production pipelines. This system integrates multiple imaging modalities including fluorescence and transmitted light (brightfield and digital phase contrast) to support diverse assay formats widely adopted in synthetic biology research and live-cell time-lapse studies.

Key technical specifications include:

• Laser Autofocus: 785 ±10 nm wavelength, 12 mW power, Class 3B laser ensuring precise and repeatable focus across varied sample types.
• Transmission Source: 740 nm LED at 80 mW for brightfield and phase contrast imaging, facilitating high-contrast visualization of cellular morphology.
• Excitation LED Light Sources:
• 365 nm UV (130 mW, 355–385 nm) for fluorophores requiring ultraviolet excitation.
• 475 nm blue (110 mW, 460–490 nm)
• 550 nm green (170 mW, 530–560 nm)
• 630 nm red (120 mW, 615–645 nm)

These multiple LED sources enable multiplexed fluorescence analysis, essential for phenotypic profiling and drug discovery screening.

• Emission Bandpass Filters: 430–500 nm, 500–550 nm (image alignment), 570–650 nm, and 655–760 nm supporting broad spectral detection aligned with common fluorescent markers used in gene editing assays and cellular biomanufacturing monitoring.

• Objective Lenses: 5×, 10×, and 20× air objectives provide flexibility for low-magnification overviews and high-resolution cellular detail critical for live-cell imaging and cell health assessment.

• Camera: High-sensitivity 4.7 Megapixel sCMOS camera delivers rapid, high-resolution image acquisition with low noise, enhancing data quality in high-throughput screening and complex imaging workflows.

The system’s compact benchtop footprint allows seamless integration in research laboratories focused on biomedical engineering and molecular biology. Its automated operation, combined with high-power LEDs and laser autofocus, assures consistent image quality and throughput necessary for synthetic biology workflows and molecular cloning pipelines.

Applications include:

• High-content cellular imaging for detailed phenotypic analysis
• Live-cell time-lapse microscopy essential for dynamic biological studies
• Drug discovery screening platforms requiring multiplexed fluorescence readouts
• Phenotypic profiling to support biotechnology and molecular medicine research

This instrument is currently sold as-is without software dongle; full testing and validation require user-provided software access. It is well-suited for labs seeking to expand high-content screening capabilities vital for biotechnological innovation and clinical diagnostics.

Relevant keywords: automated high content imaging system, fluorescence microscopy, brightfield imaging, laser autofocus system, sCMOS camera, high-throughput cellular analysis, phenotypic screening, molecular diagnostics instrumentation, biotechnology imaging platforms, biopharmaceutical production tools.