Thermo Fisher ABI QuantStudio 6 Pro Real-Time PCR System with 96-Well 0.2 mL Block

The Thermo Fisher ABI QuantStudio 6 Pro is a high-sensitivity real-time PCR system designed for quantitative PCR (qPCR) applications in molecular biology, biotechnology, and clinical diagnostics. Featuring a 96-well 0.2 mL interchangeable block with a reaction volume range of 10–100 µL, this instrument uses advanced CMOS detection technology paired with a bright white LED excitation source and a sophisticated optical system capable of monitoring up to 5–6 multiplexed targets simultaneously.

Key technical specifications include:

• Thermal Cycling Performance: Maximum ramp rate of 6.5 °C/sec and average sample ramp rate of 3.66 °C/sec with uniform temperature control within ±0.4 °C across the block, temperature range 4–99.9 °C, enabled by peltier heating/cooling technology for fast and reliable thermal profiles.
• Optical System: Five fluorescence filter combinations spanning excitation/emission wavelengths of 450–600 nm / 500–640 nm, enabling compatibility with popular qPCR dyes including FAM, SYBR Green I, VIC, JOE, HEX, TET, ABY, NED, TAMRA, ROX, Cy3, Cy5, Texas Red, Mustang Purple, and others.
• Dynamic Range and Sensitivity: Capable of detecting as low as one DNA copy with a dynamic range covering 10 orders of magnitude, delivering 1.5-fold resolution for precise quantification essential in gene expression analysis, mutation scanning, and copy number variation studies.
• Data Acquisition: Utilizes whole-plate CMOS imaging technology enabling sensitive fluorescence detection critical for multiplex qPCR assays in bioprocessing, gene editing workflows, and molecular diagnostics.
• Multiplexing Capability: Supports up to 5 or 6 simultaneous targets, facilitating complex assay designs used in molecular cloning, synthetic biology research, and biopharmaceutical production pipelines.

The system includes a heated cover to prevent condensation, communication and power cables, and is supplied with a laptop preloaded with Design & Analysis software for streamlined assay setup and data processing. Customizable block options include 96-well 0.1 mL fast block and 384-well block compatibility, adapting the instrument for diverse throughput needs.

This real-time PCR platform is widely adopted in applications such as gene expression profiling, pathogen detection, microRNA analysis, drug resistance testing, and validation of next-generation sequencing results. Its fast run times (less than 30 minutes per cycle), uniform temperature control, and multiplex fluorescent detection are critical advantages for clinical diagnostics, neuroscience research, and biomedical engineering projects requiring reliable and quantitative nucleic acid analysis.

The QuantStudio 6 Pro system is pre-owned but fully tested and serviced to ensure operational integrity with extended warranty options available. Its integration into biotechnology workflows supports demanding molecular biology experiments, including high-resolution melting (HRM) analysis and protein-DNA interaction studies like ChIP-qPCR, making it an essential tool for advanced genomic research and biomanufacturing quality control.