Bruker Berkeley Lights Beacon CAS 2.4 Premium Optofluidic Single-Cell Analysis System with Fluidics Station

The Bruker Berkeley Lights Beacon CAS 2.4 Premium is a cutting-edge optofluidic single-cell analysis system designed for precise manipulation and characterization of individual cells in biotechnology research and development. This integrated platform combines advanced microfluidic chip arrays and fluorescence imaging to enable *non-contact, light-driven positioning* of cells within nanoliter-scale NanoPen chambers (500 pL or 1 nL). The system supports high-throughput single-cell screening, essential for applications such as monoclonal antibody discovery, cell line development, T-cell and immune profiling, and functional genomics.

Key technical features include:

• OptoElectro Positioning technology for automated, light-based cell manipulation within arrays of up to 11,000 NanoPens (chip formats not included)
• Fluorescence illumination via X-Cite 120LED system (model XT120L-DMD) and 730 nm LED laser module (Thorlabs M730L4-SP1)
• Nikon motorized objective turret with Plan Fluor 10x/0.30 (DIC capable) and 4x/0.28 objectives for high-resolution brightfield and fluorescence imaging
• Five standard filter cubes (4 for fluorescence channels, 1 for brightfield/OptoElectro Positioning) enabling multiparametric analysis
• Integrated fluidics system with reagent reservoirs, manifold assembly, and an external Multi-Chip Fluidics Station for chip handling, reagent routing, and gas-assisted microfluidic control
• Precise per-chip temperature control ranging from 10°C to 40°C with Ibidi CO₂/O₂ Gas Mixer Controller for live-cell culture under controlled atmospheric conditions
• Real-time functional assays including secretion, binding, proliferation, and viability measurements with capabilities for live-cell export for downstream analysis workflows
• Compatible with Cell Analysis Suite (CAS) v2.4 Premium and Assay Analyzer 2.4 software for advanced data acquisition and analysis automation

This system is widely adopted in biotechnology and molecular diagnostics workflows, supporting *gene editing research*, *bioprocessing*, and *biopharmaceutical production pipelines*. The fluidics station uses multi-channel syringe pumps to streamline media and reagent flow, critical for maintaining physiological conditions during single-cell assays. Its modular design and automated handling are critical for synthetic biology research and cell therapy development.

Dimensions of the fully integrated unit are approximately 46 in (W) × 34 in (D) × 71.5 in (H), with a weight around 1,260 lbs. The system operates on 110–240 V AC with 50–60 Hz frequency. Although sold as-is without consumables, this instrument provides a robust platform for labs focused on personalized medicine, antibody discovery, and advanced cell profiling.

This Beacon system is essential for single-cell phenotypic screening, enabling biologists and clinical researchers to conduct comprehensive, automated experiments that were previously unattainable at high throughput. It supports seamless integration into biomanufacturing workflows and is a cornerstone technology for workflows in synthetic biology and molecular cloning applications.

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Primary Keywords included: optofluidic single-cell analysis system, fluorescence imaging, automated single-cell manipulation, microfluidic cell handling Long-tail Keywords included: light-driven cell positioning within nanoliter scale chambers, high-throughput single-cell screening for antibody discovery, automated workflows for biopharmaceutical production pipelines, critical system for cell line development and functional genomics, essential for molecular diagnostics and cell therapy research