Agilent Advanced Analytical Oligo Pro II Automated Oligonucleotide Analyzer for High-Throughput UV Sizing

The Agilent Advanced Analytical Oligo Pro II is a benchtop automated oligonucleotide analyzer designed for high-throughput nucleic acid analysis, optimized for molecular diagnostics and biotechnology workflows. This system utilizes UV absorption spectroscopy at a fixed wavelength of 254 nm for dye-free detection, enabling direct quantification and sizing of oligonucleotides up to 60-mers without fluorescent labeling.

Key features include:

• 96-capillary array for simultaneous electrokinetic injection from standard 96-well microplates, enabling complete analysis of 96 samples in approximately 1 hour.
• Compatibility with 12- and 24-capillary arrays (sold separately) for flexible throughput options.
• Automated sample processing allowing up to three 96-well plates to be analyzed consecutively without user intervention.
• Minimum sample volume of 20 µL per well, with optimum 100 µL per well.
• Direct UV absorbance detection ensures accurate purity assessment and nucleic acid integrity analysis, critical in gene editing, bioprocessing, and synthetic biology research.
• Compact dimensions (67.3 x 48.3 x 61 cm) and weight (102 kg) suitable for laboratory benchtops.

The system package includes a Dell laptop loaded with Oligo Pro II Controller Software v2.1.0.2 and Data Analysis Software v2.0.0.3, holders for skirted PCR microplates and deep-well microplates, and required fluid bottles. Note that capillary arrays are consumables with a lifespan of 1 year or 1,000 injections; replacement arrays must be purchased separately from Agilent.

This instrument is widely adopted in biotechnology sectors for applications such as oligonucleotide purity control, DNA/RNA fragment sizing and quantification, mRNA and antisense oligo analysis, and quality control in CRISPR and gene editing workflows. It is an essential tool supporting molecular diagnostics, biomanufacturing, and nucleic acid integrity assessment in clinical diagnostics and biomedical engineering research.

The instrument undergoes comprehensive functionality testing, including power, software communication, fluid handling, plate manipulation, and high-voltage operations, ensuring full operational reliability upon delivery.

Ideal for research professionals and laboratory buyers in molecular biology, biotechnology, and clinical diagnostics seeking a robust, automated solution for nucleic acid analysis with emphasis on high-throughput performance and precise UV absorbance detection.