Agilent Advanced Analytical Fragment Analyzer 5300 for High-Resolution DNA/RNA Analysis

The Agilent Advanced Analytical Fragment Analyzer 5300 is a fluorescence-based capillary electrophoresis instrument designed for sensitive, high-resolution nucleic acid analysis. It enables precise separation and quantification of DNA, RNA, and PCR fragments, with applications spanning next-generation sequencing (NGS) library preparation, molecular diagnostics, and biotechnological research.

Key technical features include:

• Fluorescence detection employing intercalating dyes for accurate sizing and quantification.
• Compatibility with both 48 and 96 capillary arrays, supporting flexible throughput options of up to 240 samples per run.
• Minimum sample requirements as low as 2.5 µL, facilitating efficient use of precious biological materials.
• High sensitivity down to 5 pg/µL for single fragments, suitable for low-abundance nucleic acid detection.
• Resolution precision capable of distinguishing fragment size differences as small as 3 base pairs for fragments under 300 bp.
• Accommodates multiple microplate formats including 96 well PCR plates (semi or non-skirted) and deep-well microplates for buffers and waste.
• Automated sample handling with three sample trays, each accommodating a full 96-well PCR plate for streamlined processing.

This medium-throughput analyzer integrates intuitive software tools (Fragment Analyzer ProSize and controller software) for streamlined data acquisition and analysis, essential for workflows in molecular cloning, gene editing verification, synthetic biology research, and biopharmaceutical production pipelines. Robust performance and precision make it widely adopted in clinical diagnostics and nucleic acid quality control, particularly for NGS library QC and cell-free DNA analysis.

The instrument’s design prioritizes flexibility and reliability, featuring a sealed capillary array system to minimize cross-contamination and wear. Its software facilitates seamless switching between DNA and RNA assays, supporting broad assay portfolios critical in biotechnology and molecular biology laboratories.

This pre-owned unit has been fully tested with comprehensive verification of power supply stability, pump operation, sample plate movement, high voltage system performance, and optical alignment to ensure maximal accuracy and reproducibility. Warranty and technical support are included, with remote installation and training options available.

Typical biotechnology applications:

• NGS library characterization
• Genomic and cell-free DNA sizing
• PCR fragment analysis
• Total RNA and in-vitro transcribed (IVT) RNA quality assessment
• Quality control in bioprocessing, molecular diagnostics, and synthetic biology research

Power specifications: 100-240 VAC, 50/60 Hz Dimensions (H x W x D): 61 cm x 38 cm x 51 cm (24” x 14” x 20”) Weight: 38.5 kg (85 lbs)

This instrument is essential for laboratories requiring robust, medium-throughput fragment analysis with high sensitivity and resolution, widely used in molecular biology, biomedical engineering, and biomanufacturing environments.