Agilent Advanced Analytical ZAG DNA Analyzer System with 96-Capillary Array for High-Throughput DNA Sizing

The Agilent Advanced Analytical ZAG DNA Analyzer System Unit 2 is a high-throughput capillary electrophoresis instrument engineered for the precise sizing and quantitation of DNA fragments and RNA samples in molecular biology workflows. This system supports a 96-capillary array configuration compatible with multiple DNA analysis kits, enabling rapid separation of up to 96 samples in approximately 20 minutes, with an unattended sample capacity of up to 864 samples and daily throughput near 4,600 samples.

Key technical features include:

• Capillary array size: 96-capillaries, supporting automated high-throughput DNA fragment analysis critical for molecular diagnostics and gene editing research.
• Sizing range: 35 base pairs (bp) to 20,000 bp, suitable for PCR fragments, microsatellites, and restriction digest analysis.
• Resolution: As low as 3 bp, gel-dependent, with sizing accuracy typically within 5%.
• Detection sensitivity: Down to 5 pg/µL DNA fragment concentration, enabling detection in various bioprocessing and synthetic biology applications.
• Sample handling: Accommodates 9 sample trays, each holding a 96-well semi or non-skirted PCR microplate with a minimum 20 µL liquid injection volume. Compatible with deep-well microplates for reagents and waste.
• Software: Integrated with ProSize data analysis and ZAG DNA Analyzer controller software for seamless data acquisition, size calling, and fluorescence analysis.
• Consumables: 96-array capillary cartridges with a 1-year or 1000-injection lifespan; replacement arrays are available from Agilent.

The system is validated through comprehensive in-house functional testing, including power supply stability, software communication, pump operation, precise plate movement, pump priming, pressure control, high voltage performance, and optical alignment of the capillary array and detection camera.

Applications of the ZAG DNA Analyzer include high-resolution DNA fragment sizing in genomics, molecular cloning workflows, synthetic biology research, and critical molecular diagnostics workflows requiring reproducible, sensitive, and rapid analysis of nucleic acids.

This compact and robust system integrates smoothly into diverse lab environments, offering biological researchers, clinical diagnostics labs, and biomanufacturing facilities a reliable solution for DNA fragment analysis essential in bioprocessing and gene editing pipelines. Remote instrument setup, training, and technical consultation are provided to ensure efficient user adoption and maintenance support.

Keywords: capillary electrophoresis DNA analyzer, high-throughput DNA fragment analysis, molecular diagnostics instrument, PCR fragment sizing, microsatellite analysis, synthetic biology DNA analysis, bioprocessing nucleic acid sizing, automated DNA analyzer, molecular biology DNA sizing platform.