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Thermo Fisher Scientific Titan Krios

UsedThermo Fisher ScientificElectron microscopesNetherlands
Procurable

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Accelerating Voltage
80–300 kV
Tilt Range
±70°
Operating Temperature
Approximately 80 K
Electron Source
Schottky X-FEG
C1 Apertures
2000, 70, 50, and 30 µm
Availability
Procurable
Lead time
Confirmed with your quote
All 20 specifications
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Specifications 20

Brands
Thermo Fisher Scientific
New/Used
Used
Accelerating Voltage
80–300 kV
Tilt Range
±70°
Operating Temperature
Approximately 80 K
Electron Source
Schottky X-FEG
C1 Apertures
2000, 70, 50, and 30 µm
C2 Apertures
150, 100, 70, and 50 µm
Objective Apertures
100, 70 µm and phase plate assembly
Acquisition Software
EPU (AFIS/FFI) Tomo
Information Limit at 300 kV at 0° / ±70° tilt
0.14 nm and 0.23 nm
Spherical Aberration (C_s)
2.7 mm
Data Collection Speed
Dependent on factors such as hole size, magnification used, and the corresponding number of movies collected per hole. With standard AFIS data collection, the more movies per stage movement that can be collected, the faster the data acquisition rate will be.
Fringe-free Illumination
Upgraded in Krios I, II, III, and IV at eBIC, which increases data collection speeds by allowing more movies per hole due to the reduced illumination sizes used.
Number of Cartridges
12
Sample Exchange Time
Approximately 3 minutes
Imaging Filter and Detector
TFS Selectris X and Falcon 4i 4096 x 4096, 14 µm pixels, 320 fps max frame rate (eer) DQE@ 0.5NF= 0.72
Retractable Bottom Mount Detector
FEI Falcon III. 4096 x 4096, 14 µm pixels, 38/39 fps max frame rate DQE@ NF/2 > 0.5
Availability
Procurable
Lead time
Confirmed with your quote

Product description

300 kV cryo-TEM with automated sample loading and advanced detectors for high-resolution biological imaging.

Titan Krios is a state-of-the-art 300 kV cryogenic transmission electron microscope (cryo-TEM) designed for high-resolution imaging of biological specimens in their native, hydrated states. Since its introduction in 2009, this instrument has been pivotal in advancing structural biology research.

Key features include:

  • Automated sample loading: Robotic handling of up to 12 frozen-hydrated samples enhances throughput and minimises contamination.
  • High-resolution imaging: Equipped with advanced detectors like the Gatan K3 and Falcon 4i, it delivers exceptional image quality for detailed structural analysis.
  • Versatile applications: Supports single-particle analysis, electron tomography, and 2D electron crystallography, facilitating comprehensive studies of molecular and cellular structures.
  • Stability and automation: Features such as ConstantPower™ lenses and automated liquid nitrogen refilling ensure prolonged, stable operation with minimal user intervention.

The Titan Krios is extensively utilised in structural biology, molecular biology, and biomedical research for applications like drug discovery, vaccine development, and disease mechanism elucidation. It is a cornerstone instrument in university laboratories, biomedical research centres, and pharmaceutical companies worldwide.

By providing unparalleled insights into the three-dimensional architecture of biological macromolecules, the Titan Krios continues to drive significant advancements in life sciences.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 8 November 2026