Cytiva ImageQuant LAS 4000

The ImageQuant LAS 4000 is a high-performance digital imaging system designed for sensitive, quantitative imaging of gels and blots, utilising both chemiluminescence and fluorescence detection methods. This biomolecular imager is essential for researchers requiring precise quantitation and documentation of Western, Northern, and Southern blots.

Key features include:

• Wide linear dynamic range: Accurately quantifies both weak and strong signals over four orders of magnitude within a single image, eliminating the need for multiple exposures.
• High resolution: Provides precise quantitation of gels and films up to 21 × 14 cm, achieving up to 6.3 megapixel resolution through a unique diagonal pixel configuration.
• Low noise: The camera, typically cooled to -25°C (maximum -35°C), enables longer exposure times with reduced background noise, crucial for detecting very weak signals in chemiluminescent Western blotting.
• High sensitivity: Capable of detecting fluorescent and chemiluminescent signals down to picogram levels of target protein, with sensitivity enhancements available through pixel binning up to 8 × 16.
• Uniform quantitation: Applies distortion, dark frame, and flat frame corrections to each imaging mode, ensuring optimal precision.
• Multifluorescence detection: The system can be equipped to image a wide range of UV- and RGB-excited labels and stains, enhancing its versatility.

The ImageQuant LAS 4000 is widely utilised in various scientific fields, including molecular biology, biotechnology, and biomedical research. It is commonly employed in university laboratories, biomedical research centres, and clinical environments for applications such as Western blot analysis, gel documentation, and fluorescence imaging.

First released in the early 2010s, the ImageQuant LAS 4000 remains a relevant and valuable tool in laboratories, offering reliable performance for a range of imaging applications.