Agilent 5200 Fragment Analyzer Unit 2 for Fluorescence-Based DNA Fragment Analysis

The Agilent 5200 Fragment Analyzer Unit 2 is a sophisticated capillary electrophoresis instrument designed for fluorescence-based DNA and RNA fragment analysis widely used in molecular diagnostics, genomics research, and biotechnology applications including gene editing and bioprocessing workflows. Utilizing a 12-capillary array, the system performs parallel nucleic acid separation with high sensitivity and resolution, capable of detecting fragment differences as small as 3 base pairs for fragments under 300 bp and sensitivities down to 5 pg/µL.

Key technical specifications include:

• Sample throughput: Processes up to 288 samples per run across three 96-well PCR microplates, ideal for medium-throughput laboratory environments.
• Sample volume requirements: 20 µL liquid injection volume with as little as 1 µL actual sample used, optimizing precious biological materials.
• Applications supported: Next-generation sequencing (NGS) fragment analysis, plasmid and large DNA fragment sizing, genomic DNA quality control, microsatellite/SSR genotyping, reverse genetics, and TILLiNG mutation detection.
• Analysis method: Fluorescence-based capillary electrophoresis utilizing intercalating dyes for detailed sizing and quantification.
• Accessories included: Marker/sample PCR microplate holders, deep-well microplate holders, waste bottles, and a control computer with proprietary ProSize data analysis software. (Note: Capillary arrays and consumables are not included.)
• Operational reliability: The unit undergoes rigorous functional validation including power supply stability, pump operation, optical alignment of capillary arrays, plate handling accuracy, fluidics prime testing, and high-voltage system verification.
• Automation features: Automated wash and fluid handling with precise pump control minimize user intervention and enhance reproducibility, critical for sensitive molecular biology workflows.
• User support: Available remote instrument setup, training sessions, and ongoing technical sales consultation to facilitate integration into biopharmaceutical production pipelines and synthetic biology research labs.

This instrument is critical for biotechnology laboratories focusing on molecular cloning, gene editing verification, genomic fragment sizing, and PCR product analysis, supporting workflows in clinical diagnostics and biomedical engineering research. The Agilent 5200’s robust design and extensive throughput options make it popular among researchers requiring high-precision nucleic acid fragment analysis with reliable performance and minimal maintenance.

Keywords incorporated naturally: capillary electrophoresis DNA fragment analyzer, fluorescence-based nucleic acid analysis, medium-throughput genomics, molecular diagnostics instrumentation, gene editing fragment sizing, bioprocessing fragment analysis, SSR genotyping system, synthetic biology research tool.

This model is part of Agilent’s current generation instruments, sold with a 120-day warranty in the continental US and 60-day international warranty, with shipping available worldwide and supported by established servicing expertise.