Agilent 5200 Fragment Analyzer - Capillary Electrophoresis System for High-Precision DNA Fragment Analysis

The Agilent 5200 Fragment Analyzer is a fluorescence-based capillary electrophoresis instrument designed for high-precision DNA and RNA fragment analysis. Widely used in molecular diagnostics, genomics research, and biotechnology applications such as bioprocessing and synthetic biology, this automated system offers low- to mid-throughput nucleic acid separation essential for fragment sizing, concentration measurement, and mutation detection workflows.

Key features include:

• Analysis Method: Fluorescence-based capillary electrophoresis utilizing intercalating dyes for sensitive detection.
• Capillary Array: Supports a 12-capillary array enabling simultaneous parallel analysis of up to 12 samples per run.
• Sample Throughput: Accommodates up to 288 samples per run using 3 x 96-well PCR microplates, ideal for medium-throughput molecular biology labs.
• Sample Volume: Minimum of 20 µL liquid injection volume with as little as 1 µL actual sample required, optimizing precious samples in molecular cloning or gene editing research.
• Resolution and Accuracy: Resolves fragment size differences as small as 3 bp for fragments below 300 bp, providing precise sizing critical for microsatellite (SSR) genotyping, plasmid DNA analysis, and NGS fragment quality control.
• Sensitivity: Detects DNA/RNA down to 5 pg/µL enabling reliable detection in low abundance samples.
• Sample Handling: Compatible with semi or non-skirted 96-well PCR plates for marker/sample analysis and deep-well plates for buffers and waste.
• Automation & Maintenance: Includes automated wash system and long-life capillaries reducing user intervention and minimizing downtime, which is critical in demanding biomanufacturing or molecular diagnostic workflows.

The analyzer is engineered for diverse applications such as NGS fragment analysis, large genomic DNA and plasmid fragment sizing, SSR genotyping, and mutation screening via TILLiNG, making it a versatile tool across clinical diagnostics, synthetic biology research, and biotechnological innovation. The controlled pump and pressure system, along with validated optical alignment and high voltage stability, ensure reproducible electrophoretic separations and robust data quality.

This system comes with a robust hardware and software package, including the Fragment Analyzer controller and ProSize data analysis software, facilitating data acquisition and interpretation vital for quality control in nucleic acid bioprocessing and gene editing pipelines. The Agilent 5200 supports laboratories needing efficient, reproducible fragment analysis with minimized risk of sample cross-contamination or instrument errors.

In summary, the Agilent 5200 Fragment Analyzer is a critical asset for biotechnology and genomics laboratories requiring precise nucleic acid fragment sizing and quantification, widely adopted for its reliability in synthetic biology workflows, molecular cloning, and clinical genomic research.