Leica DMi8 Stellaris 5 Motorized Inverted Confocal Fluorescence Microscope with AFC Module

The Leica DMi8 Stellaris 5 Confocal Laser Scanning Fluorescence Microscope System integrates a fully motorized inverted research microscope platform with advanced fluorescent and confocal imaging modalities, tailored for biotechnology, cell biology, and biomedical engineering workflows.

Key technical features include:

• Microscope Base: Leica DMi8 inverted microscope equipped with CTRadvanced control box and Stellaris 5 scanning head with AOBS (Acousto-Optical Beam Splitter) technology enhancing spectral resolution and reducing phototoxicity.
• Motorized Components: External fluorescence axis, 6-position fluorescence filter cube turret, 6-position objective turret, 7-position condenser with rotatable polarizer, motorized scanning stage (Marzhauser), motorized Z-axis focus, automated adaptive focus control, and motorized illumination intensity control for LED and laser sources.
• Fluorescence Illumination: Combines Leica LED 3 Light Engine (Lumencor) and Stellaris 5 white laser (485–790 nm) with 405 nm diode laser for UV excitation, supporting multiparameter fluorescence imaging across up to 8 laser lines, enabling essential molecular diagnostics and live-cell fluorescence applications.
• Optics and Objectives: High-performance HC PL APO objectives (10x to 63x magnification, numerical apertures up to 1.40 Oil), motorized objective turret allows rapid switching for detailed subcellular imaging, critical in bioprocessing and synthetic biology research.
• Imaging Capabilities:
• Confocal laser scanning with variable scanning resolution (16×16 up to 2048×2048) facilitating both fast overview and high-resolution imaging.
• Multidimensional imaging including 3D volumetric reconstruction, Z-stacks, time series, and multi-position acquisition.
• Integrated Leica LAS X software featuring advanced modules such as 3D Advanced Basic and Full, Deconvolution Expert, Dye Finder for fluorophore optimization, and Assay Editor to automate complex imaging protocols.
• Automated stitching for large-area high-content imaging workflows.
• Environmental Control: Tokai Hit STXG stage-top incubator for live-cell imaging with temperature and CO₂ regulation, enhancing reproducibility in dynamic cellular studies and biomanufacturing process monitoring.
• Camera and Detection: Stellaris 5 hybrid detectors (HyD) optimized for low-noise fluorescence detection, ensuring high sensitivity critical for molecular biology and gene editing assays.
• Applications: The system supports advanced research in molecular diagnostics, biopharmaceutical production pipelines, synthetic biology, live-cell dynamics, fluorescence colocalization studies, tissue biomarker analysis, neuroimaging, and materials science.
• Customization: Options for higher magnification objectives, additional fluorescence filter cubes and sets, upgrade to high-performance CCD, sCMOS, or EMCCD cameras, alternate fluorescence light sources (xenon, metal halide, LED), motorized shutters, and expanded environmental control. The microscope can accommodate diverse sample formats including slides, Petri dishes, and microplates.
• Service & Warranty: Professionally serviced with preventive maintenance completed, optics verified for field distortion and emission uniformity, inclusion of new fluorescence filters, 180-day USA and 60-day international warranty, plus remote training on microscope operation and software.
• Biotechnology Optimization: The AOBS technology provides sharp spectral resolution with minimal photobleaching, essential for sensitive fluorescent probes used in gene editing and molecular cloning workflows. The system’s motorized automation and software-controlled operation enable reproducible imaging protocols, critical in bioprocessing and biomanufacturing research environments.

This Leica DMi8 Stellaris 5 system represents a versatile platform combining precision motorized optics and advanced confocal fluorescence capabilities, widely adopted in synthetic biology, molecular diagnostics, and live-cell imaging studies requiring quantitative and high-resolution microscopy.