Thermo Scientific Applied Biosystems QuantStudio 7 Pro Real-Time PCR System with 384-Well Block

The Thermo Scientific Applied Biosystems QuantStudio 7 Pro Real-Time PCR System with 384-well block is engineered for robust high-throughput quantitative PCR (qPCR) workflows essential in modern molecular diagnostics and biotechnology research. It supports a wide variety of nucleic acid quantification applications including gene expression analysis, SNP genotyping, pathogen detection, methylation studies, and validation of next-generation sequencing (NGS) data.

Key Technical Specifications:

• Block Format: Interchangeable with installed 384-well block optimized for high-throughput analysis
• Sample Capacity: 384 wells accommodating reaction volumes from 5 to 20 µL
• Thermal Cycling Performance:
• Maximum ramp rate: 6.0 °C/sec
• Average sample ramp rate: 2.92 °C/sec
• Temperature range: 4–99.9 °C with uniformity of ±0.4 °C
• Detection System: Advanced CMOS whole-plate imaging technology paired with bright white LED excitation light source
• Multiplexing Capability: Supports up to 5 or 6 fluorescent targets simultaneously, critical for multiplex gene expression and genotyping assays
• Dynamic Range & Sensitivity: 10 orders of magnitude dynamic range and 1.5-fold resolution with detection sensitivity down to a single copy, enabling precise molecular quantification
• Optical Filters and Dye Compatibility: 21 color and filter combinations compatible with common fluorophores such as FAM, SYBR Green I, VIC, JOE, HEX, TET, TAMRA, Cy3, ROX, Cy5, and others widely used in molecular biology and biotechnology assays
• Heating/Cooling Technology: Peltier-based thermal control for rapid and uniform thermal cycling
• Physical Dimensions: 52.5 x 33.8 x 54.7 cm; weight: 38 kg

Applications:

• Quantitative PCR (qPCR) for gene expression, microRNA profiling, and copy number variation analysis
• High-Resolution Melting (HRM) analysis for mutation scanning and epigenetics research
• Pathogen detection and viral load quantification critical in clinical diagnostics and infectious disease research
• Methylation analysis for epigenetic modifications
• Drug resistance and genotyping assays in pharmacogenomics and molecular diagnostics
• Protein-DNA interaction studies (e.g., ChIP-qPCR) and RNA expression profiling

Additional Features:

• User-friendly touchscreen interface with customizable experimental settings for research flexibility
• Short run times (<30 minutes) enhance laboratory throughput and efficiency in bioprocessing and molecular cloning workflows
• Optional interchangeable blocks (96-well fast and standard formats, plus 384-well) for versatile assay designs
• Comprehensive dye calibration and preventative maintenance performed, ensuring accuracy and instrument reliability

Widely adopted in synthetic biology, biopharmaceutical production pipelines, and molecular diagnostics laboratories, the QuantStudio 7 Pro Real-Time PCR system combines high-throughput capabilities with precision thermal cycling and sensitive fluorescence detection. Its design supports essential workflows in gene editing, biomanufacturing, and biomedical engineering, making it a critical asset for advanced life science research and clinical applications.

---

Keywords integrated: Real-Time PCR system, quantitative PCR instrument, high-throughput qPCR, molecular diagnostics, gene expression analysis, multiplex PCR, fluorescence detection, thermal cycler, bioprocessing, synthetic biology research, molecular cloning workflows, biopharmaceutical production, clinical diagnostics, next-generation sequencing validation, Peltier thermal control, microscopy.