Qiagen Rotor-Gene Q 5Plex Real-Time PCR System with Rotary Thermal Cycling

The Qiagen Rotor-Gene Q 5Plex Real-Time PCR System is a precision rotary thermal cycler designed for quantitative PCR workflows requiring exceptional temperature uniformity and multiplex detection performance. Utilizing a unique spinning rotor mechanism, it eliminates edge effects and well-to-well variation common in block-based qPCR instruments, ensuring reproducible amplification results, critical in molecular diagnostics and bioprocessing research.

Key specifications include:

• Thermal Cycling: Temperature accuracy ±0.5ºC, resolution ±0.02ºC, range 35–99ºC, with rapid ramp rates >15ºC/s heating and >20ºC/s cooling for efficient cycling.
• Optical Detection: Supports up to 6 fluorescence channels from UV to near-infrared, employing high-energy LEDs and photomultiplier detectors for sensitive multiplex qPCR assays, including 5-plex detection capability.
• Sample Capacity: Accommodates up to 100 samples per run using Rotor-Disc 100 format and is compatible with 0.1 ml tubes and strip tubes, ideal for medium-throughput molecular biology and clinical applications.
• Dynamic Range: 10 orders of magnitude, enabling detection from low to high template concentrations, essential for gene expression studies, mutation analysis, and pathogen detection.
• Environmental and Power: Operates at 18–30ºC with 10–75% noncondensing humidity; power requirements 100–240 V AC, 50–60 Hz.

This benchtop system is widely adopted in biotechnology, molecular diagnostics, synthetic biology, and biomanufacturing workflows, where reliable multiplex PCR and quantitative reproducibility are paramount. The Rotor-Gene Q is essential for labs conducting gene expression analysis, copy number variation studies, and pathogen genotyping. It supports validated assays, enabling seamless integration into existing PCR workflows without extensive revalidation or retraining.

Included accessories enhance operational readiness, such as a Rotor with Locking Cap (72 x 0.1 ml tubes), Windows 10 notebook with Rotor-Gene Q Pure Detection Software, communication cables, and power cords. This system provides a compact footprint and established performance metrics, making it a preferred choice for researchers and clinical scientists seeking consistent, high-throughput real-time PCR instrumentation.