Qiagen QIAcuity Four Digital PCR System with Multiplex Nanoplate Technology

The Qiagen QIAcuity Four Digital PCR System is an advanced, fully integrated digital PCR platform designed for molecular diagnostics and biotechnology research. It processes up to four 8-, 24-, or 96-well nanoplates simultaneously, enabling high-throughput absolute quantification critical in applications such as gene expression analysis, rare mutation detection, and pathogen quantification.

Key features include:

• Parallel processing of up to four nanoplates for high sample throughput (up to 768 samples/day using 96-well plates)
• Nanoplate-based partitioning with thousands of partitions for precise detection of low-abundance targets
• Multiplexing capability with 5-plex fluorescence detection across green, yellow, orange, red, and crimson channels, aiding complex gene panel and mutation analysis
• Integrated thermal cycling with a temperature range of 35–99 °C, a ramp rate of approximately 3 °C/s, ±1 °C temperature accuracy and homogeneity ensuring consistent amplification
• High-resolution image acquisition via a 6.3 MP CMOS camera combined with excitation from a high-power white LED (4750 lumens) for sensitive endpoint fluorescence detection
• Optimized for walk-away workflows with minimal handling, supporting reproducibility and efficiency in molecular biology workflows such as bioprocessing, molecular diagnostics, and synthetic biology research
• Power requirements: 100–240 VAC, 50/60 Hz, 1000 VA

The system supports critical biotechnological applications including:

• Rare mutation detection and copy number variation analysis relevant to oncology and molecular genetics
• Gene expression quantification for transcriptomics studies
• Pathogen detection and viral load measurement in clinical diagnostics and biomanufacturing

The QIAcuity Four’s integrated workflow combines partitioning, thermal cycling, and imaging in a single instrument, widely adopted among molecular biology researchers for precision and scalability. Its nanoplate technology and multiplex fluorescence capabilities make it essential for advanced digital PCR assays in biopharmaceutical production pipelines and synthetic biology research.

This unit is from 2022, fully serviced, includes a laptop with QIAcuity software, power and communication cables, and comes with warranty and after-sales technical support. Shipping is available worldwide with proper insurance and expert packing to preserve instrument integrity during transit.

This digital PCR system is particularly suited for researchers engaged in biotechnology, clinical diagnostics, and molecular biology needing precise quantification and high throughput in digital PCR workflows.